Molecular characterization of carbapenem-resistant Acinetobacter baumannii isolates causing bloodstream infections in intensive care unit

Basit Öğe Kaydı
dc.contributor.authorGözalan, Ayşegül
dc.contributor.authorÜnaldı, Özlem
dc.contributor.authorKırca, Fisun
dc.contributor.authorÇöplü, Nilay
dc.contributor.authorMüderris, Tuba
dc.contributor.authorAçıkgöz, Ziya Cibali
dc.contributor.authorDurmaz, Rıza
dc.date.accessioned2021-02-19T21:20:49Z
dc.date.available2021-02-19T21:20:49Z
dc.date.issued2020
dc.departmentALKÜ
dc.description.abstractObjective: In this study, the aim was to investigate the clonal relationship between carbapenem resistant Acinetobacter baumannii isolates and carbapenem resistance genes isolated from blood samples of patients followed in intensive care units by molecular methods. Methods: Bactec 9240 system (Becton Dickinson, USA) was used for the isolation of bacteria from blood culture flasks. Identification of 112 strains included in the study were performed by conventional tests, API 20NE (bioMerieux, France) and Phoenix TM 100 system (Becton Dickinson, USA) and confirmed by the presence of blaOXA-51 gene. Antimicrobial susceptibility tests were performed by Kirby-Bauer disk diffusion method and Phoenix TM 100 system. Carbapenem resistance genes; blaOXA-23, blaOXA-48, blaOXA-58, blaIMP, blaVIM and blaNDM-1 were investigated by Multiplex Polymerase Chain Reaction (PCR) method. Pulsed Field Gel Electrophoresis (PFGE) was used to determine the clonal relationship between Acinetobacter baumannii strains. Results: The antibiotic resistance percentages of strains for gentamicin, amikacin, tobramycin, netil micin, ceftazidime, trimethoprim/sulfamethoxazole, piperacillin, ciprofloxacin, ampicillin/sulbactam, piperacillin/tazobactam and cefoperazone/ sulbactam, were 88%; 81%; 78%; 36%; 98.5%; 96%; 89%; 100%; 100%; 93%; 91% respectively. MIC values of imipenem and meropenem were determined above ?8 ?g/ml in the whole group. blaOXA-51 and blaOXA-23 genes were detected in all isolates included in the study. By PFGE method, 62 different pulsotypes were detected. Among the pulsotypes, 19 of them contained ?2 strains. It was observed that 108 (96.4%) strains were clustered in 11 clonally related groups when the similarity between pulsotypes for grouping was limited to 85% or more. Conclusion: In this study, it was observed that carbapenem-resistant A. baumannii strains were resistant for all tested antibiotics at high levels except netilmicin and spread in the hospital via cross contamination. These strains posed a risk for hospital infections, however, clonal-related strains were not limited to a specific unit and time period. © 2020 Refik Saydam National Public Health Agency (RSNPHA.
dc.identifier.doi10.5505/TurkHijyen.2019.53323
dc.identifier.endpage24en_US
dc.identifier.issn0377-9777
dc.identifier.issue1en_US
dc.identifier.scopusqualityQ4
dc.identifier.startpage15en_US
dc.identifier.urihttps://doi.org/10.5505/TurkHijyen.2019.53323
dc.identifier.urihttps://hdl.handle.net/20.500.12868/713
dc.identifier.volume77en_US
dc.indekslendigikaynakScopus
dc.institutionauthor0-belirlenecek
dc.language.isotr
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.relation.ispartofTurk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAcinetobacter baumannii
dc.subjectAntibiotic resistance
dc.subjectIntensive care units
dc.subjectPolymerase chain reaction
dc.subjectPulsed-field gel electrophoresis
dc.titleMolecular characterization of carbapenem-resistant Acinetobacter baumannii isolates causing bloodstream infections in intensive care unit
dc.title.alternativeYoğun bakim ünitelerinde kan dolaşimi enfeksiyonu etkeni karbapenem dirençli Acinetobacter baumannii izolatlarinin moleküler yöntemlerle karakterizasyonu
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu