Evaluation of Cytotoxic, Membrane Damaging and Apoptotic Effects of Origanum majorana Essential Oil on Lung Cancer and Epidermoid Carcinoma Cells

Basit Öğe Kaydı
dc.contributor.authorGokhan, Ayse
dc.date.accessioned2026-01-24T12:01:03Z
dc.date.available2026-01-24T12:01:03Z
dc.date.issued2022
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractBACKGROUND/AIMS: In this study, the potential of an essential oil to cause membrane damaging and induce apoptosis was investigated in order to determine the cytotoxic effect of the essential oil obtained from Origanum majorana (O. majorana), popularly known as “sweet marjoram”, on lung cancer (A-549) and epidermoid carcinoma (A-431) cells. MATERIALS AND METHODS: In order to determine the cytotoxic dose and IC50 value of an essential oil, the essential oil was applied to cells in the concentration range of 5-500 ?g/mL for 24, 48 and 72 hours. After those applications, CellTiter-Blue® Cell Viability Assay and 3-(4,5-Dimethyl- 2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay were performed on the cell lines. The lactate dehydrogenase (LDH) Activity test was used to determine the membrane damaging effect of the essential oil on A-549 and A-431 cells. Cytotoxicity experiments were followed up by caspase-3/7 activity assays to get a mechanistic insight into the associated molecular pathways. RESULTS: It was observed that treatments of essential oil from O. majorana on A-549 and A-431 cells inhibited cell proliferation, that is, it had a cytotoxic effect and caused an increase in LDH activity, which is one of the membranes damaging markers, and caspase-3/7 activity, which is one of the enzymes involved in the apoptotic pathway, in both cells in comparison to controls at the end of the 24-hour incubation. CONCLUSION: The results of this study indicate that the increase in LDH activity and caspase-3/7 activity caused by O. majorana essential oil treatment reveal the membrane damaging and apoptotic effects, respectively. O. majorana essential oil can be evaluated as a potential therapeutic agent for lung cancer and epidermoid carcinoma due to these effects. However, more research is needed to confirm this.
dc.identifier.doi10.4274/cjms.2021.2021-142
dc.identifier.endpage206
dc.identifier.issn2149-7893
dc.identifier.issn2536-507X
dc.identifier.issue2
dc.identifier.startpage201
dc.identifier.trdizinid1121876
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1121876
dc.identifier.urihttps://doi.org/10.4274/cjms.2021.2021-142
dc.identifier.urihttps://hdl.handle.net/20.500.12868/3937
dc.identifier.volume7
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofCyprus Journal of Medical Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260121
dc.subjectEssential oil
dc.subjectOriganum majorana
dc.subjectapoptotic effect
dc.subjectmembrane damage
dc.titleEvaluation of Cytotoxic, Membrane Damaging and Apoptotic Effects of Origanum majorana Essential Oil on Lung Cancer and Epidermoid Carcinoma Cells
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu