Analysis of mitochondrial DNA cytochrome-b (CYB) and ATPase-6 gene mutations in COVID-19 patients

Basit Öğe Kaydı
dc.contributor.authorDirican, Ebubekir
dc.contributor.authorSavrun, Şeyda Tuba
dc.contributor.authorAydın, İsmail Erkan
dc.contributor.authorGülbay, Gonca
dc.contributor.authorKaraman, Ülkü
dc.date.accessioned2022-09-12T10:29:47Z
dc.date.available2022-09-12T10:29:47Z
dc.date.issued2022
dc.departmentALKÜ, Fakülteler, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü
dc.description.abstractCoronavirus disease of 2019 (COVID?19) is a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS?CoV?2). Mutations of mitochondrial DNA (mtDNA) are becoming increasingly common in various diseases. This study aims to investigate mutations in the cytochrome?b (CYB) and adenosine triphosphatase?6 (ATPase?6) genes of mtDNA in COVID?19 patients. The association between mtDNA mutations and clinical outcomes is investigated here. In the present study, mutations of the mtDNA genes CYB and ATPase?6 were investigated in COVID?19 (+) (n = 65) and COVID?19 (?) patients (n = 65). First, we isolated DNA from the blood samples. After the PCR analyses, the mutations were defined using Sanger DNA sequencing. The age, creatinine, ferritin, and CRP levels of the COVID 19 (+) patients were higher than those of the COVID?19 (?) patients (p = 0.0036, p = 0.0383, p = 0.0305, p < 0.0001, respectively). We also found 16 different mutations in the CYB gene and 14 different mutations in the ATPase?6 gene. The incidences of CYB gene mutations A15326G, T15454C, and C15452A were higher in COVID?19 (+) patients than COVID?19 (?) patients; p < 0.0001: OR (95% CI): 4.966 (2.215?10.89), p = 0.0226, and p = 0.0226, respectively. In contrast, the incidences of A8860G and G9055A ATPase?6 gene mutations were higher in COVID?19 (+) patients than COVID?19 (?) patients; p < 0.0001: OR (95%CI): 5.333 (2.359?12.16) and p = 0.0121 respectively. Yet, no significant relationship was found between mtDNA mutations and patients' age and biochemical parameters (p > 0.05). The results showed that the frequency of mtDNA mutations in COVID?19 patients is quite high and it is important to investigate the association of these mutations with other genetic mechanisms in larger patient populations.
dc.identifier.doi10.1002/jmv.27704
dc.identifier.endpage3146en_US
dc.identifier.issue7en_US
dc.identifier.startpage3138en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12868/1552
dc.identifier.volume94en_US
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.relation.ispartofJournal of Medical Virology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectATPase?6
dc.subjectCOVID?19
dc.subjectCYB
dc.subjectmtDNA
dc.subjectPCR
dc.subjectSanger sequencing
dc.titleAnalysis of mitochondrial DNA cytochrome-b (CYB) and ATPase-6 gene mutations in COVID-19 patients
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Journal of Medical Virology - 2022 - Dirican - Analysis of mitochondrial DNA cytochrome‐b CYB and ATPase‐6 gene mutations.pdf
Boyut:
1.65 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam metin / Full text
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu