The effect of aging and exercise training on carbon monoxide relaxation response in thoracic aorta and gastrocnemius feed artery
Yükleniyor...
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmanın amacı yaşlanma ve yüzme egzersizine bağlı olarak sıçanların torasik aortasında ve gastroknemius iletim arterinde karbon monoksit (CO)’e verilen damar yanıtlarının değişip değişmediğini test etmektir. Yöntem: Çalışmamızda 4 aylık genç ve 24 aylık yaşlı Wistar dişi sıçanlar kullanıldı. Sıçanlar rastgele gençsedanter (GS), genç-egzersiz (GE), yaşlı-sedanter (YS) ve yaşlı-egzersiz (YE) olmak üzere dört gruba ayrıldı. Egzersiz gruplarına 8 hafta süresince haftada 5 gün, günde 1 saat yüzme egzersizi yaptırıldı. Sıçanlardan izole edilen torasik aorta halkaları organ banyosu düzeneğinde, gastroknemius iletim arteri ise telli miyograf düzeneğinde çalışıldı. Aort ve gastroknemius iletim arteri halkalarının fenilefrin (PE) kasılma yanıtları hemoksijenaz inhibitörü chromium mesoporphyrin inkübasyonu öncesi ve sonrasında kaydedilerek endojen olarak üretilen CO’in vasküler tonusa katkısı değerlendirildi. CO donörü (CORM; carbon - monoxide - releasing - molecule) kullanılarak ekzojen gevşeme yanıtları değerlendirildi. Bulgular: CrMP inkübasyonu öncesinde ve sonrasında PE doz yanıt eğrilerinde hem aortada hem de gastroknemius iletim arterinde gruplar arasında fark yoktu. CrMP inkübasyonu sonrasında alınan maksimum PE kasılma yanıtları aort halkalarında GE, YS ve YE gruplarında anlamlı olarak daha yüksekti (p<0,05), gastroknemius iletim arterlerinde ise sadece GS ve GE gruplarında anlamlı olarak yüksekti (p<0,01). CORM’a verilen maksimum gevşeme yanıtlarında gastroknemius iletim arterinde gruplar arasında fark yoktu. Aorta halkalarında ise maksimum CORM gevşeme yanıtları yaşlı gruplarda anlamlı olarak daha düşüktü (p<0,05). Sonuç: Çalışmanın sonuçları; gastroknemius iletim arterinde CO yanıtlarına egzersiz ve yaşlanmanın etkisi olmadığını, Aorta halkalarında ise CO gevşeme yanıtının yaşlanmayla birlikte azaldığını ve egzersizin CO gevşeme yanıtını hem yaşlı hem de genç sıçanlarda arttırıcı bir katkısının olmadığını göstermektedir.
Objective: The purpose of this study is to observe any aging and swimming exercise associated changes in the rat thoracic aorta and gastrocinemius feed artery. Methods: 4-month and 24-month old female rats were used and divided into four following groups: sedentary young, trained young, sedentary old, and trained old. Swimming exercise was performed for 8 weeks (60 min/day, 5 days/week). Thoracic Aorta and gastrocinemius feed artery isolated from the rats. Thoracic Aorta rings were mounted on organ bath and gastrocinemius feed arteries were mounted on wire myograph. Contraction responses of all vessel rings in presence and absence of HO inhibitor (CrMP) were recorded as an endogenous CO contribution to vascular tonus. The effect of exogenous CO relaxation response were assesed by CO releasing molecule (CORM). Results: Phenylephrine dose- response curves with or without CrMP were smilar in all groups both aortic rings and gastrocnemius feed artery. The maximum PE contraction responses obtained after CrMP incubation were significantly higher in the GE, YS and YE groups (p <0.05) in the aortic rings, and only significantly higher in the GS and GE groups in the gastrocnemius feed arteries (p <0,01). There was no differences in the maximum vasodilatation response of CORM reported for all groups in gastrocnemius feed artery. However, in the old groups CORM Emax values were established significantly lower than that the young groups in the aortic rings (p<0,05). Conclusion: As a results of this study; exercise and aging have no effect on CO responses in the gastrocnemius feed artery. But the CO relaxation response is decreased by aging in the thoracic aorta of elderly rats and exercise does not have an enhancing contribution for CO relaxation response in both old and young rats.
Objective: The purpose of this study is to observe any aging and swimming exercise associated changes in the rat thoracic aorta and gastrocinemius feed artery. Methods: 4-month and 24-month old female rats were used and divided into four following groups: sedentary young, trained young, sedentary old, and trained old. Swimming exercise was performed for 8 weeks (60 min/day, 5 days/week). Thoracic Aorta and gastrocinemius feed artery isolated from the rats. Thoracic Aorta rings were mounted on organ bath and gastrocinemius feed arteries were mounted on wire myograph. Contraction responses of all vessel rings in presence and absence of HO inhibitor (CrMP) were recorded as an endogenous CO contribution to vascular tonus. The effect of exogenous CO relaxation response were assesed by CO releasing molecule (CORM). Results: Phenylephrine dose- response curves with or without CrMP were smilar in all groups both aortic rings and gastrocnemius feed artery. The maximum PE contraction responses obtained after CrMP incubation were significantly higher in the GE, YS and YE groups (p <0.05) in the aortic rings, and only significantly higher in the GS and GE groups in the gastrocnemius feed arteries (p <0,01). There was no differences in the maximum vasodilatation response of CORM reported for all groups in gastrocnemius feed artery. However, in the old groups CORM Emax values were established significantly lower than that the young groups in the aortic rings (p<0,05). Conclusion: As a results of this study; exercise and aging have no effect on CO responses in the gastrocnemius feed artery. But the CO relaxation response is decreased by aging in the thoracic aorta of elderly rats and exercise does not have an enhancing contribution for CO relaxation response in both old and young rats.
Açıklama
Anahtar Kelimeler
Karbon monoksit, Hemoksijenaz, Vasküler tonus, Aorta, Gastroknemius iletim arteri, Carbon monoxide, Hemo oxygenase, Vascular tonus, Gastrocnemius feed artery
Kaynak
Turk Hijyen ve Deneysel Biyoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
77
Sayı
4
Künye
Bağlantı
https://www.scopus.com/record/display.uri?eid=2-s2.0-85101506420&origin=resultslist&sort=plf-f&src=s&nlo=&nlr=&nls=&sid=20c4fe371b9b908d4f7e419f791331ff&sot=aff&sdt=cl&cluster=scofreetoread%2c%22all%22%2ct&sl=72&s=AF-ID%28%22Alanya+Alaaddin+Keykubat+University%22+60198720%29+AND+SUBJAREA%28MEDI%29&relpos=165&citeCnt=0&searchTerm=
https://hdl.handle.net/20.500.12868/2449
https://hdl.handle.net/20.500.12868/2449
