Screening of cytotoxicity and DNA topoisomerase IIa inhibitory activity of Turkish Onosma species

Basit Öğe Kaydı
dc.contributor.authorGüzel, Özge
dc.contributor.authorDuman, Seda
dc.contributor.authorYilmaz, Sinem
dc.contributor.authorKarakoyun, Çiğdem
dc.contributor.authorKul, Demet
dc.contributor.authorPirhan, Ademi Fahri
dc.contributor.authorBedir, Erdal
dc.date.accessioned2026-01-24T12:20:46Z
dc.date.available2026-01-24T12:20:46Z
dc.date.issued2021
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractOnosma L., the largest genus of Boraginaceae, is represented by 105 species in Turkey with an endemism rate of 52%. Phytochemical studies indicate that Boraginaceae plants mainly comprise naphthoquinones with a wide range of biological activities including anticancer, antiinflammatory, wound healing, and antioxidant effects. However, few taxa of the genus Onosma have been investigated in detail for their bioactivities. Considering the high rate of endemism and an inadequate number of bioactivity screening studies in literature, we aimed to evaluate the cytotoxic effects and topoisomerase inhibitory activities of some Onosma species growing in southwestern Turkey. Here, we describe a comprehensive cytotoxic activity screening study on petroleum ether, dichloromethane, and methanol extracts of the roots of 20 identified and one unidentified Onosma taxa. The MTT cell viability assay has been performed to investigate the cytotoxicity of the extracts against seven cancer cell lines (MCF-7, HeLa, Hep G2, A549, Capan-1, HCC-1937, and DU-145) and a noncancerous cell line (MRC-5), while doxorubicin was served as a positive standard. The petroleum ether extracts of O. aksoyii Aytaç&Türkmen, O. isaurica Boiss. and Heldr., O. taurica Pallas ex Willd. var. taurica and O. alborosea Fisch. & C.A. Mey subsp. alborosea var. alborosea were determined as the most active ones based on their IC<inf>50</inf> values. DNA topoisomerase II? inhibition assay was conducted on the petroleum ether and dichloromethane extracts of these four active species, and almost all tested extracts demonstrated strong inhibition on the enzyme at a concentration of 0.1 mg/mL. Our cytotoxicity screening results were consistent with the findings of the topoisomerase II? inhibition test. This study advocates the significant role of Onosma species in the field of anticancer drug discovery. © TÜBİTAK.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (116Z463); Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK
dc.identifier.doi10.3906/bot-2108-23
dc.identifier.endpage788
dc.identifier.issn1300-008X
dc.identifier.issueSI2
dc.identifier.scopus2-s2.0-85132845626
dc.identifier.scopusqualityQ2
dc.identifier.startpage776
dc.identifier.urihttps://doi.org/10.3906/bot-2108-23
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4568
dc.identifier.volume45
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTurkiye Klinikleri
dc.relation.ispartofTurkish Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20260121
dc.subjectbioactivity
dc.subjectcytotoxic
dc.subjectendemic
dc.subjectOnosma
dc.subjectscreening
dc.subjecttopoisomerase
dc.titleScreening of cytotoxicity and DNA topoisomerase IIa inhibitory activity of Turkish Onosma species
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu