Investigation of Toll-Like Receptor Family Expression in Glioblastoma: A Comparative Analysis of qPCR and Cell Culture

Basit Öğe Kaydı
dc.contributor.authorŞahin, Sevinç
dc.contributor.authorSabah, Seda
dc.contributor.authorElmas, Levent
dc.contributor.authorHacisalihoglu, Uguray Payam
dc.contributor.authorYanık, Serdar
dc.date.accessioned2026-01-24T12:01:24Z
dc.date.available2026-01-24T12:01:24Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractAim: Glioblastoma is the most frequent, and fatal brain cancer in adults. Toll-like receptors are cell surface receptors comprised of 10 receptors (Toll-like receptor 1?10) related to triggering innate immunity by recognizing pathogens. However, some studies suggested that the expression of Toll-like receptors might be related to cancer cell proliferation and progression in some tumors including glioblastoma with some contradictory results. Thus, we aimed to investigate all ten members of the Toll-like receptor expression profile in glioblastoma for the first time in the literature to contribute additional data to the literature. Patients and Methods: Quantitative real-time polymerase chain reaction was applied to formalin-fixed paraffin-embedded tissues of 25 glioblastoma patients, diagnosed between January and December 2018, to evaluate the mRNA expression of Toll-like receptors. Also, the expression of each Toll-like receptor was investigated by cell culture analysis using five different cell lines of human glioblastoma (T98G, U87-MG, U373, LN18, and A172). The results were compared statistically. Results: Toll-like receptor 1, Toll-like receptor 6, and Toll-like receptor 7 mRNA levels were significantly increased in the formalin-fixed paraffin-embedded tissues of the glioblastoma group (p<0.001, each) whereas the expression of Toll-like receptor 4 and Toll-like receptor 10 was downregulated compared to the control group (p=0.023, p<0.001, respectively), by quantitative real-time polymerase chain reaction analysis. Additionally, Toll-like receptor mRNA expression profiles differed among the cell lines. Conclusion: In our study, many Toll-like receptor members seemed to display different expression level in the glioblastoma microenvironment and affect it diversely. Further comprehensive studies are required to confirm the endogenous protein level of each Toll-like receptor in glioblastoma, to clarify their precise role in the pathogenesis and prognosis of glioblastoma, and to shed light on new target therapies.
dc.identifier.doi10.30733/std.2023.01603
dc.identifier.endpage68
dc.identifier.issn1017-6616
dc.identifier.issn2149-8059
dc.identifier.issue2
dc.identifier.startpage62
dc.identifier.trdizinid1183454
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1183454
dc.identifier.urihttps://doi.org/10.30733/std.2023.01603
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4298
dc.identifier.volume39
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofSelçuk Tıp Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TR-Dizin_20260121
dc.subjectBiyoloji,Nörolojik Bilimler,Onkoloji,Biyokimya ve Moleküler Biyoloji
dc.titleInvestigation of Toll-Like Receptor Family Expression in Glioblastoma: A Comparative Analysis of qPCR and Cell Culture
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu