Effect of combination treatment of protocatechuic acid with 5-fluorouracil and oxaliplatin on colon cancer Caco-2 cell line

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dc.authorid0000-0002-3555-3946
dc.contributor.authorYildiz, Fatma
dc.contributor.authorEciroglu, Hamiyet
dc.contributor.authorOvey, Ishak Suat
dc.contributor.authorAvnioglu, Seda
dc.date.accessioned2026-01-24T12:26:46Z
dc.date.available2026-01-24T12:26:46Z
dc.date.issued2023
dc.departmentAlanya Alaaddin Keykubat Üniversitesi
dc.description.abstractAmong the most common antitumor drugs used in the treatment of colon cancer are 5-fluorouracil and oxaliplatin (5-FU and OXA). However, both these drugs have many side effects, and hence there is a need for new treatment\approach to reduce the side effects aas well as drug concentration. In this context, here, we investigated the effect of addition of protocatechuic acid (PCA) onto either monotherapies or combination therapies of 5-FU and OXA on the human colon cancer (Caco-2) cell line. In addition, we did evaluate the synergistic effect of PCA with 5-FU and OXA. Further, we determined the suppressive effects of different doses of PCA alone or in combination with 5-FU/OXA on cell proliferation after 24 and 48 hours. We identified a suppressive effect of PCA on cell viability at 48 h starting from the dose of 50 mu M Matrix metalloproteinase-2 (MMP-2) and MMP-9 gene expression levels and apoptotic effects showed significant increases and decreases depending on the dose and time applied in the experimental groups. The highest synergistic activity was seen at 2:1 concentration of 5-FU+ PCA. Our findings indicate the presence of the cytotoxic and apoptotic effects of PCA in Caco-2 cells at 48 h, increasing with a dose-and time-dependent manner.
dc.description.sponsorshipScientific Research Projects of Alanya Alaaddin Keykubat University [2019-15-01-MAP01]
dc.description.sponsorshipAcknowledgement This research was supported by the Scientific Research Projects of Alanya Alaaddin Keykubat University (Project number 2019-15-01-MAP01) .
dc.identifier.doi10.56042/ijeb.v61i03.65610
dc.identifier.endpage158
dc.identifier.issn0019-5189
dc.identifier.issn0975-1009
dc.identifier.issue3
dc.identifier.startpage151
dc.identifier.urihttps://doi.org/10.56042/ijeb.v61i03.65610
dc.identifier.urihttps://hdl.handle.net/20.500.12868/4926
dc.identifier.volume61
dc.identifier.wosWOS:000946576800001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherNatl Inst Science Communication-Niscair
dc.relation.ispartofIndian Journal of Experimental Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20260121
dc.subjectAnticancer activity
dc.subjectCaco-2
dc.subjectCombination index
dc.subjectDose reduction index (DRI)
dc.subjectMatrix metalloproteinases (MMP)
dc.titleEffect of combination treatment of protocatechuic acid with 5-fluorouracil and oxaliplatin on colon cancer Caco-2 cell line
dc.typeArticle

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