Yazar "Gök, Mehmet Koray" seçeneğine göre listele

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    Çift Tabakalı Hidroksit ile Oksalik Asit Uzaklaştırılmasının Modellenmesi ve Optimizasyonu
    (Alanya Alaaddin Keykubat Üniversitesi, 2024) Şentürk, Sema; Gamsızkan, Halil; Gök, Mehmet Koray; Aşçı, Yavuz Selim; Gök, Aslı
    Bu çalışmanın temel amacı, oksalik asit (OxA) adsorpsiyon koşullarının çift tabakalı hidroksit (LDH) kullanılarak optimizasyonunun araştırılması, adsorpsiyonun hem tepki yüzeyi metodolojisi (RSM) hem de yapay sinir ağı (YSA) ile modellenmesidir. Mg-Al LDH, birlikte çöktürme yöntemiyle sentezlenmiş olup, Fourier dönüşümlü kızılötesi spektroskopisi (FTIR), İndüktif eşleşmiş plazma - kütle spektrometresi (ICP-MS) ve X-ışını kırınımı (XRD) teknikleriyle karakterize edilmiştir. Adsorpsiyon proses tasarımının gerçekleştirilmesi için gerekli olan denge süresi ve kinetik model verileri incelenmiştir. OxA uzaklaştırma yüzdesi ölçülürken bağımsız değişkenler olarak proses süresi, başlangıç asit konsantrasyonu, sıcaklık ve adsorban dozajı seçilmiştir. Bu sonuçların hem RSM hem de YSA teknikleriyle modellenmesi, RSM modelinden biraz daha iyi bir belirleme katsayısı gösteren bir YSA modeliyle sonuçlanmıştır. Modeller prosesin optimal koşulları için tutarlı sonuçlar vermiştir.
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    Öğe
    Investigation of the Usability of Polymeric Nanoparticle-Based Transfection Agent for CRISPR/CAS9 in HUVEC Cell Line
    (2025) Imamoglu, Rızvan; Kaplan, Ozlem; Gök, Mehmet Koray; Gokce, Isa
    Genome editing technology is a promising, popular technology for diagnosing and treating many diseases. One of the most popular techniques utilized in genome editing research nowadays is the CRISPR/Cas system. The CRISPR/Cas system holds considerable potential for diagnostic and therapeutic applications due to its ease of use, speed, and affordability. The safe and effective delivery of the CRISPR-based genome editing system to the intended human body cells is one of the system's problems. This study investigated the silencing of the COX-2 gene via the CRISPR/Cas system using a P?AE-based transfection agent in the HUVEC cell line. Western blot analysis was performed to confirm COX-2 gene silencing in the HUVEC cell line, and the effects of COX-2 gene silencing on cell migration in these cells were examined. As a result, the transfection efficiency of the P?AE-based transfection agent for the HUVEC cell line was determined as 90-95%, and the CRISPR efficiency was calculated as 57.12% based on the Western blot results. It was revealed that the P?AE-based nanoparticles used in this study can be used as a good transfection agent in CRISPR/Cas-mediated genome editing in the HUVEC cell line, especially in plasmid transfection.
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    Öğe
    Optimization Studies of Mg/Al-NO3 Layered Double Hydroxide Nanoparticles by Hydrothermal Treatment
    (Alanya Alaaddin Keykubat University, 2023) Bal, Kevser; Şentürk, Sema; Kaplan, Özlem; Gök, Mehmet Koray; Pabuccuoğlu, Saadet Kevser
    Layered Double Hydroxides based nanoparticles offer significant advantages in biological applications with high biocompatibility and low cytotoxicity. In this study, nanoparticles (nMg/Al-NO3-LDH) were synthesized by the co-precipitation method and synthesis optimization of the nanoparticles was carried out by hydrothermal treatment. The effect of hydrothermal treatment on Z-average and surface charge was evaluated. Experiments were performed at 80°C and 100°C during in the range of 2-48 h by using different stirring rates (250, 1000, and 1500 rpm) and without stirring. Dynamic Light Scattering (DLS) was used to assess the particle size (nm), polydispersity index (PDI), and zeta potential (mV) of the nanoparticles. The chemical structure of nanoparticles was characterized by Fourier Transform Infrared spectrometry (FTIR). As a result, nanoparticles with an optimum particle size of 86.87 nm, a PDI of 0.132 and a zeta potential (mV) of 44.4±8.74 were obtained at 80°C, 48h and 250 rpm. The data showed that Mg/Al-NO3-LDH nanoparticles have suitable physical properties for biological applications.