Yazar "Erdogan, Ayse" seçeneğine göre listele

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    Carvacrol enhances the sensitıvity of cetuximab in lung cancer cells through inducing apoptosis and cell cycle arrest
    (Bangladesh Pharmacological Soc, 2025) Erdogan, Ayse; Koras, Rahime Aybike
    The intention of this work was to analyze that carvacrol could enhance cancer-inhibiting potency of cetuximab (monoclonal antibody) in lung cancer cells. The combination of cetuximab and carvacrol was found to co-operatively suppress cell proliferation by enhancing apoptosis and inducing cell cycle arrest in A-549 and H1299 cells. Furthermore, the combination therapy triggered cell death by inducing membrane disruption. The most effective combinations were IC10 cetuximab + IC10 carvacrol for A-549 and IC20 cetuximab + IC10 carvacrol for H1299 (CI = 2.0). LDH activity increased by 101% in A-549 and 239% in H1299 (p<0.05). Caspase-3 activity rose by 1.6-fold in A549 and 1.4-fold in H1299 (p<0.05). The combination decreased PCNA, topoisomerase II-alpha, and cyclins D1, D2, E, A, and B (p<0.05). Overall, the combination of carvacrol and cetuximab appears to enhance anti-cancer efficacy and may significantly improve therapeutic outcomes in lung cancer cells.
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    Combined Low-Dose Cetuximab and Green Tea Epigallocatechin-3-Gallate Treatment Induces Lung Cancer Cell Death
    (Springer, 2025) Erdogan, Ayse
    Green tea catechins, especially epigallocatechin-3-gallate (EGCG), have been associated with cancer prevention and treatment. Cetuximab is a chimeric monoclonal antibody that is directed toward the epidermal growth factor receptor (EGFR). The use of EGCG could enhance the effect of cetuximab through additive or synergistic effects as well as through amelioration of deleterious side effects. Cytotoxic, antiproliferative, cell-cycle-inhibitory, oxidative, and apoptotic effects of cetuximab alone and together with EGCG on A-549 and H1299 cells were investigated. EGCG enhanced cytotoxic effect of cetuximab on all cells. Lactate dehydrogenase activity, as a result of cell death, was found to be at the highest level at treatment of cetuximab with EGCG in all cells. Treatment of cetuximab with EGCG was found to be more effective at increasing glutathione peroxidase activity than cetuximab alone. Proliferating Cell Nuclear Antigen (PCNA), topoisomerase II-alpha, cyclin D1, cyclin D2, cyclin E, cyclin A, and cyclin B gene expression was decreased in all cells, which explains the cell-cycle-inhibitory effect. Both cetuximab alone and in combination treatments caused an increase in the Bax/Bcl-2 ratio in both type of cell. These findings suggest that treatment of cetuximab combined with EGCG might exhibit more carcinogenesis-reducing potential than cetuximab alone.
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    Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells
    (2025) Erdogan, Ayse
    The determination that compounds used for chemotherapeutic purposes in cancer treatment cause cytotoxic effects on healthy cells as well as target cells has led researchers to work on reducing treatment-related toxicity, and accordingly, research on the effectiveness of herbal resources in cancer treatment has gained importance. In our study, ethanol, methanol and chloroform extracts were obtained from the aboveground parts of Origanum majorana L. (O. majorana) and their cytotoxic effects on A-549 cells (non-small cell lung cancer (NSCLC)) were investigated using MTT test. Additionally, the free radical scavenging activities of extracts were demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. It has been shown that cytotoxic effects of extracts increased depending on concentrations on A-549 cells. It was determined that methanol, ethanol and chloroform extracts of O. majorana caused an increase in the activity of lactate dehydrogenase (LDH), and apoptotic enzyme, caspase-3 activity, compared to the control. It has been demonstrated that methanol, ethanol, and chloroform extracts of O. majorana had membrane damaging and apoptotic effects on A-549.
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    Comparison of the cytotoxic effects of different Origanum majorana extracts on lung cancer cells
    (Marmara Univ, Fac Pharmacy, 2025) Erdogan, Ayse
    The determination that compounds used for chemotherapeutic purposes in cancer treatment cause cytotoxic effects on healthy cells as well as target cells has led researchers to work on reducing treatment-related toxicity, and accordingly, research on the effectiveness of herbal resources in cancer treatment has gained importance. In our study, ethanol, methanol and chloroform extracts were obtained from the aboveground parts of Origanum majorana L. (O. majorana) and their cytotoxic effects on A-549 cells (non-small cell lung cancer (NSCLC)) were investigated using MTT test. Additionally, the free radical scavenging activities of extracts were demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. It has been shown that cytotoxic effects of extracts increased depending on concentrations on A-549 cells. It was determined that methanol, ethanol and chloroform extracts of O. majorana caused an increase in the activity of lactate dehydrogenase (LDH), and apoptotic enzyme, caspase-3 activity, compared to the control. It has been demonstrated that methanol, ethanol, and chloroform extracts of O. majorana had membrane damaging and apoptotic effects on A-549.
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    Effects of Epigallocatechin Gallate on the Cytotoxicity of Epirubicin-HCl in Lung Cancer Cells
    (Bentham Science Publ Ltd, 2023) Erdogan, Ayse; Ozkan, Aysun
    Background: Studies have shown that epigallocatechin-3-gallate (EGCG), which is present in green tea at a higher rate than other components, has an additive or synergistic cytotoxic effect when applied with different anticancer drugs and reduces the side effects caused by anticancer drugs. It is known that the order of administration of drugs in combined applications also affects cytotoxicity. In this context, our study determined the most effective application sequence by evaluating the cytotoxic responses of epirubicin-HCl and EGCG according to the different application orders in A-549 cells (NSCLC). Methods: To demonstrate the apoptotic activity, we detected changes in mRNA ratios of Bax, a pro-apoptotic gene, and Bcl-2, an anti-apoptotic gene (Bax/Bcl2), as well as changes in the activity of caspase 3/7 enzyme. To demonstrate the effect of oxidative stress generation, we investigated changes in glutathione peroxidase activity. Results: It was observed that the cell viability of A-549 cells exposed to different concentrations of epirubicin-HCl and EGCG for 48 h decreased depending on the concentration increase. According to the results of cell viability in cells to which epirubicin-HCl (
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    Evaluation of Cytotoxic and Apoptotic Effects of Methotrexate and Carvacrol Combination in Lung Cancer Cancer Cells
    (2025) Erdogan, Ayse
    The aim of this study is to compare the cytotoxic and apoptotic effects of methotrexate (MTX),one of chemotherapy drug, alone application with the combined application of MTX and carvacrol, a naturally occurring monoterpenoid phenol, in lung cancer cells. Cell viability was assessed using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay after A-549 cells were treated with MTX alone and in combination with carvacrol for 48 h. Lactate dehydrogenase (LDH) activity test was employed to assess whether MTX alone or in combination with carvacrol treatments, induced membrane damage by detecting changes in LDH activity in A-549 cells. Caspase-3 enzyme activity changes in cells were measured to show the apoptotic effect. By examining the alterations in glutathione peroxidase activity, an attempt was made to determine whether MTX, alone and in combination with carvacrol, played a role in inducing oxidative stress. It was observed that the combined application of MTX and carvacrol for 48 h inhibited cell proliferation more than MTX alone treatment, demonstrating a stronger cytotoxic effect. Additionally, it caused a greater increase in LDH activity, a marker of membrane damage, and also led to a higher increase in caspase-3 activity, an enzyme involved in the apoptotic pathway. The results of this study show that the combined application of MTX and carvacrol leads to a greater increase in LDH activity and caspase-3 activity compared to MTX alone treatment, indicating more significant membrane damage and apoptotic effects. Therefore, combined treatment could be considered a strategy for alleviating the side effects associated with MTX in lung cancer.
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    Evaluation of the effect of a natural monoterpenic phenol on the cytotoxicity of carfilzomib
    (2024) Erdogan, Ayse
    Aim: The aim of this study was to reveal whether carfilzomib, proteasome inhibitor, and carvacrol, a natural monoterpenic phenol, causes cytotoxic and apoptotic effects and oxidative stress on A-549 cells. Materials and Methods: Lactate dehydrogenase (LDH) activity test was used. Changes in caspase 3 and glutathione peroxidase enzyme activities in cells were determined. Results: It was determined that carfilzomib alone and together with carvacrol caused a raise in the activities of lactate dehydrogenase (LDH), glutathione peroxidase and apoptotic enzyme, caspase-3 activity, compared to the control. Conclusion: Our study showing that carfilzomib alone and together with carvacrol gave different responses may be guiding in determining new strategies to be applied in lung cancer treatment.
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    Evaluation of the Effect of Thymol on the Cytotoxicity of Cetuximab in Lung Cancer Cells
    (2025) Erdogan, Ayse; Haznedar, Ekin; Başer, Mehmet
    The treatment of lung cancer continues to be a significant challenge for many oncologists and their patients. Treatment using epidermal growth factor receptor inhibitors is connected to a positive outcome. Cetuximab, a chimeric monoclonal antibody targeting the epidermal growth factor receptor (EGFR), in conjunction with monoterpene phenol thymol, is recommended for the treatment of lung cancer. While a mild acne-like skin rash is quite frequent in patients using cetuximab, a severe rash is rare. The goal of the current study was to assess whether thymol could enhance the anticancer effectiveness of cetuximab in A-549, non-small cell lung cancer (NSCLC) cell line. We found that the combination of cetuximab and thymol synergistically suppressed cell proliferation by inducing membrane damaging, oxidative stress, and apoptosis in A-549 cells. Taken together, our results indicate that the combination of thymol and cetuximab could improve anticancer responses and may notably enhance treatment outcomes in NSCLC.
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    Uçucu Yağların ve Bileşenlerinin Antikanser Etki Mekanizmalarının Değerlendirilmesi
    (2025) Erdogan, Ayse
    Antikanser ajan olarak yeni etken madde olarak doğal bitkisel ürünlerin araştırılması günümüzde çok hızlı ilerleyen ve oldukça dikkat çeken araştırma konularından biridir. Son yıllarda, uçucu yağların kanser tedavisindeki etkilerini değerlendiren çalışmaların sayısı önemli ölçüde artmış ve hız kazanmıştır. Uçucu yağ bileşenleri arasında monoterpenler, seskiterpenler, oksijenli monoterpenler, oksijenli seskiterpenler ve fenolikler bileşikler yer almaktadır. Bu derlemede farklı kanser hücrelerindeki uçucu yağların ve bileşenlerinin hangi hücresel etki mekanizmaları kullanarak antikanser ajan olarak değerlendirilebileceğini gösteren çalışmalara değinilmiştir. Uçucu yağlar ve bileşenlerinin kanser hücrelerini apoptoza yönlendirerek, hücre döngüsünü duraksatarak, antimetastatik ve antianjiyogenik etki göstererek, reaktif oksijen türlerinin ve reaktif azot türlerinin (ROT/RNS) miktarını arttırarak ve DNA tamir mekanizmalarını uyararak antiproliferatif aktivite gösterdikleri yapılan çalışmalar ile ortaya konmuştur. Uçucu yağ ve bileşenlerinin Süperoksit dismutaz (SOD), katalaz, glutatyon peroksidaz ve glutatyon reduktaz gibi detoksifikasyon enzimlerinin aktiviteleri üzerinde de etki gösterdikleri yapılan çalışmalarla gösterilmiştir.