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Öğe Antipsychotics Induced Reproductive Toxicity by Stimulating Oxidative Stress: A Comparative in Vivo and in Silico Study(Wiley-V C H Verlag Gmbh, 2023) Dincer, Busra; Bulent Yazici, Ahmet; Cinar, Irfan; Toktay, Erdem; Selli, Jale; Cadirci, Elif; Bayraktutan, ZaferThe pathophysiological mechanism behind the link between antipsychotic drugs and sexual dysfunction is still unknown. The goal of this research is to compare the potential effects of antipsychotics on the male reproductive system. Fifty rats were randomly assigned into the five groups indicated: Control, Haloperidol, Risperidone, Quetiapine and Aripiprazole. Sperm parameters were significantly impaired in all antipsychotics-treated groups. Haloperidol and Risperidone significantly decreased the level of testosterone. All antipsychotics had significantly reduced inhibin B level. A significant reduction was observed in SOD activity in all antipsychotics-treated groups. While GSH levels diminished, MDA levels were rising in the Haloperidol and Risperidone groups. Also, the GSH level was significantly elevated in the Quetiapine and Aripiprazole groups. By causing oxidative stress and altering hormone levels, Haloperidol and Risperidone are damaging to male reproductivity. This study represents useful starting point for exploring further aspects of the underlying mechanisms reproductive toxicity of antipsychotics.Öğe Effects of soy isoflavonoids (genistein and daidzein) on endometrial receptivity(Mashhad Univ Med Sciences, 2020) Toktay, Erdem; Selli, Jale; Gürbüz, Muhammed Ali; Taştan, Tuğba Bal; Uğan, Rüstem Anıl; Un, Harun; Halıcı, ZekaiObjective(s): This study aimed to examine the effects of genistein and daidzein on endometrial receptivity by histopathological, immunohistochemical, and biochemical techniques. Materials and Methods: In this study, 72 female Sprague-Dawley rats were randomly divided into 8 groups. The endometrial receptivity model was applied to identified groups. Experimental animals were given periorally 10 mg/kg and high 40 mg/kg doses of genistein and daidzein for 5 days by gavage. At the end of the experiment, uterine tissues were evaluated histopathologically, immunohistochemically, and biochemically. Results: When histopathological findings were examined, significant decreases in pinopod formation were observed in high dose genistein and daidzein groups. When compared with the endometrial receptivity group, immunohistochemical staining findings showed a significant decrease in the expression of integrin beta 3, integrin alpha v beta 3, LIF, and HOXA10 and an increase in MUC 1 expression in the high dose of genistein and daidzein groups. In biochemical evaluations, it was determined that genistein and daidzein increased estrogen levels and decreased progesterone levels in a dose-dependent manner. Conclusion: Genistein and daidzein have a negative effect on endometrial receptivity. Therefore, individuals with a risk of infertility should pay attention to the consumption of genistein and daidzein.Öğe Histopathological Overview of Experimental Ulcer Models(2022) Toktay, Erdem; Selli, JaleHistopathology is the process of examining tissue that includes all the changes, when a diseased tissue shows compared to a healthy group with a result of a histological observation. Histopathology has become an essential process in medical experimental research and medical experimental models. Scientists have developed medical experimental animal models for these reasons and have pioneered new drug research for many years. One of these experimental researches is experimental ulcer models. This model, which was initially a single method, has led to the emergence of new models with the discovery of physiological processes on ulcers by scientists. Nowadays, researchers have performed many new peptic ulcer models on experimental animals over the years. The main point in the creation of the ulcer model is the increase in the stomach acid level and the removal or corruption of the gastric mucus. When the experimental models were examined histopathologically, it was seen that the most severe models were those induced by pyloric ligation, acetic acid application, and indomethacin. In these models, ulcer foci that progressed to the submucosa were common, while the superficial damage spreading to the entire surface was striking in the ethanol model. While epithelial losses are shown on the surface of the mucosa, foci of necrotic apoptotic cell clusters extending to the submucosa are shown according to the weight of the model. In addition, evidence of inflammation has been shared in almost all studies. All these results show that ulcer models can be created by many different mechanisms. However, similar findings were observed in almost all experiments. Whether the experimental model caused severe or mild ulceration changed the histological findings.Öğe Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats(Mashhad Univ Med Sciences, 2023) Toktay, Erdem; Selli, Jale; Gurbuz, Muhammet Ali; Alaca, RaziyeObjective(s): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques.Materials and Methods: Seventy two Sprague-Dawley female rats with an average weight of 200-250 gr and 10-12 weeks old were randomly divided into 9 groups. PCOS model was applied to all groups except healthy group. In the study, low (10 mg / kg) moderate (20 mg / kg) and high (40 mg / kg) doses of ASX were given to the experimental animals in the PCOS-induced groups for 7 days. At the end of the experiment, ovarian tissues were evaluated histopathologically, immunohistochemically, and biochemically. Results: When the histopathological findings were examined, many cystic follicles, apoptotic and necrotic cells were found in the follicles in the PCOS group. In addition, significant decrease in apoptotic and necrotic cells were observed in PCOS+MET+ASX and PCOS+ASX groups. In immunohistochemical staining findings, while TNF-alpha, NF-KB and IL-6 expression levels showed significant increase in PCOS group, these expression levels were decreased in PCOS+MET+ASX and PCOS+ASX groups. In the biochemical evaluations, while MDA were increased, SOD were decreased in the PCOS group. MDA level were decreased while SOD levels were increased in the PCOS+MET+ASX and PCOS+ASX groups.Conclusion: In addition to the formation of insulin resistance in the tissue, severe oxidative stress damage occurs in ovarian tissue during PCOS. Metformin improved PCOS by correcting insulin resistance. In this period, the administration of ASX with Metformin protected the ovary from oxidative stress damageÖğe Nicotinamide Riboside Preserves Ovarian Injury in an Experimental Sepsis Model in Rats(2023) Selli, Jale; Dilek, Vural Keleş; Keleş, Osman Nuri; Çelik, Muhammet; Yetim, ZelihaObjective: The aim of the study is to investigate the protective effects of nicotinamide riboside on oxidative stress in an experimental sepsis model created by cecal ligation and puncture. Materials and Methods: Rats were divided into 3 groups randomly: sham-operated (control) group, sep sis group, and nicotinamide riboside-treated group. Sepsis model-induced cecal ligation and puncture was applied to sepsis group rats. Animals in the nicotinamide riboside-treated group were administered nicotin amide riboside intraperitoneally (500 mg/kg). Tissue specimens from rats were biochemically calculated for their activities of catalase, superoxide dismutase, glutathione peroxidase, myeloperoxidase, and malondialde hyde levels. Ovarian tissues of all rats were histopathologically evaluated. Results: Catalase, superoxide dismutase, and glutathione peroxidase activities were lower in the sepsis group compared to the sham-operated (control) group. Superoxide dismutase activity was significantly higher in the nicotinamide riboside-treated group than in control and sepsis group (P < .05). Myeloperoxidase activi ties and mean malondialdehyde concentration of ovarian tissue were lower in nicotinamide riboside-treated group than in sepsis group (P < .05). The light microscopic assessment revealed that ovarian tissue was protected, and inflammation and interstitial edema decreased in nicotinamide riboside-treated group. The follicular damage findings were notably decreased in nicotinamide riboside-treated group in comparison to sepsis group (P<0.05). Conclusion: Our findings indicated that nicotinamide riboside diminished ovarian injury in sepsis via inhibiting tissue infiltration and increasing endogenous antioxidant capacity. Nicotinamide riboside administration may represent a new treatment approach for the prevention of sepsis-induced ovarian injuryÖğe Nicotinamide Riboside Preserves Ovarian Injury in an Experimental Sepsis Model in Rats(Aves, 2023) Selli, Jale; Keles, Dilek Vural; Keles, Osman Nuri; Celik, Muhammet; Yetim, ZelihaObjective: The aim of the study is to investigate the protective effects of nicotinamide riboside on oxidative stress in an experimental sepsis model created by cecal ligation and puncture. Materials and Methods: Rats were divided into 3 groups randomly: sham-operated (control) group, sepsis group, and nicotinamide riboside-treated group. Sepsis model-induced cecal ligation and puncture was applied to sepsis group rats. Animals in the nicotinamide riboside-treated group were administered nicotinamide riboside intraperitoneally (500 mg/kg). Tissue specimens from rats were biochemically calculated for their activities of catalase, superoxide dismutase, glutathione peroxidase, myeloperoxidase, and malondialdehyde levels. Ovarian tissues of all rats were histopathologically evaluated. Results: Catalase, superoxide dismutase, and glutathione peroxidase activities were lower in the sepsis group compared to the sham-operated (control) group. Superoxide dismutase activity was significantly higher in the nicotinamide riboside-treated group than in control and sepsis group (P < .05). Myeloperoxidase activities and mean malondialdehyde concentration of ovarian tissue were lower in nicotinamide riboside-treated group than in sepsis group (P < .05). The light microscopic assessment revealed that ovarian tissue was protected, and inflammation and interstitial edema decreased in nicotinamide riboside-treated group. The follicular damage findings were notably decreased in nicotinamide riboside-treated group in comparison to sepsis group (P<0.05). Conclusion: Our findings indicated that nicotinamide riboside diminished ovarian injury in sepsis via inhibiting tissue infiltration and increasing endogenous antioxidant capacity. Nicotinamide riboside administration may represent a new treatment approach for the prevention of sepsis-induced ovarian injury.Öğe Potential protective effect of astaxanthin on ovary ischemia-reperfusion injury(2022) Toktay, Erdem; Taştan, Tuğba Bal; Gürbüz, Muhammet Ali; Erbaş, Elif; Demir, Özlem; Ulgan, Rüstem Anıl; Selli, JaleObjective(s): We thought that astaxanthin (ASX) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat ovary. Materials and Methods: The experimental groups consisted of healthy, I (Ischemia), I+ASX50, I+ASX100, I/R (Ischemia/Reperfusion), I/R+ ASX50, and I/R+ ASX100. Vascular clamps were applied to the ovaries for 3 hr to induce ischemia. For the reperfusion groups, the clamps were opened and blood flow was restored to the ovaries for 3 hr. At the end of the experiment, biochemical, histopathological, and immunohistochemical analyses were made from the tissue samples taken. Results: While MDA levels increased significantly in I and I/R groups, SOD levels decreased. It was found that ASX significantly decreased MDA levels and increased SOD activity in treatment groups depending on the dose. Caspase 3, IL-1 ?, and IL-6 expressions were severely increased in ischemia and I/R groups, while the severity of I+ASX50 and I/R+ASX100 immunoreactivity was decreased. While severe hemorrhage areas were observed in I and IR groups, minimal hemorrhage areas were observed in the treatment groups, especially in I/R+ASX100 groups. In addition, inflammatory cells and necrotic cells in the I/R group were not observed in I/R+ASX50 and I/R+ASX100 groups. Conclusion: As a result, it was determined that ASX has a strong protective role against oxidative damage in the treatment of ovarian ischemia-reperfusion injuryÖğe Ratlarda Clp (Cecal Ligation And Puncture) Ile Oluşturulan Sepsis Modelinde Ebselenin Etkilerinin Incelenmesi(2016) Erkayman, Beyzagul Polat; Çadırcı, Elif; Selli, Jale; Bayraktutan, Zafer; Albayrak, Abdulmecit; Akpınar, Erol; Ün, HarunSepsis ve sepsise bağlı komplikasyonlar ve ölüm günümüz tıp bilim alanının hala daha çözemediği sorunların başında gelmektedir. Sepsiste birçok ilaç kullanılmakta olup gerçek radikal tedavi hala daha bulunamamıştır. Sepsiste ölüme neden olan en son hasar akciğerde gerçekleşmekte ve solunum durması ve ileri derecede akciğer hasarı sepsisin en önemli komplikasyonlarını oluşturmaktadır. Selenyum bileşiklerinin çok güçlü antioksidan ve antiinflamatuvar özellikleri oldukları bilinmektedir ve deneysel olarak çeşitli akciğer hasarlanmalarında yararlı bulunmuşlardır. Bu nedenle bu proje kapsamında, deneysel olarak sıçanlarda oluşturulan sepsis modelinde bir selenyum bileşiği olan ebselenin sepsiste yüksek mortalite ve morbiditenin en önemli nedeni olan akciğer hasarını önlemedeki etkinliği ve etki mekanizması araştırılıldı. Çalışmamızda sepsis modeli, insanda oluşan sepsise en yakın olan çekal ligasyon ve puncture (CLP) yöntemi ile yapılarak bir selenyum bileşiği olan ebselen intraperitoneal yoldan ve hemen deney öncesinde uygulandı. 16 saat boyunca hayvanlar gözlenerek, mortalite oranları hesaplandı. Deney sonunda biyokimyasal, moleküler ve histopatolojik incelemeler yapılması amacı ile ratların akciğeri çıkarılarak kanları alındı. Sonuç olarak deneysel sepsis modelinde bir selenyum bileşiği olan ebselenin mortalite oranlarını düşürdüğü, sıçanların akciğer dokularında koruyucu etkiler oluşturduğu belirlendi.
