Yazar "Savaş, Hasan Basri" seçeneğine göre listele

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    Öğe
    A Mathematical modelling approach in the spread of the novel 2019 Coronavirus SARS-CoV-2 (COVID-19) pandemic
    (Modestum Ltd, 2020) Çakır, Zafer; Savaş, Hasan Basri
    The novel 2019 coronavirus, SARS-CoV-2 (COVID-19), emerged towards the end of 2019 in the city of Wuhan in the province of Hubei in the People's Republic of China, and it has spread to the entire world very fast and in a very short time. This study aimed to investigate the course of the pandemic by mathematical modelling based on the information that the time-dependent change (spreading) rate of the H number of individuals who have caught a contagious disease is proportional to the multiplication of the numbers of those who have caught the disease and those who have not. According to the results of the mathematical modelling in our study, in the case that sufficient precautions are not taken, or precautions are reduced, the course of the pandemic may show a very fast change in the negative direction. For this reason, every precaution, individual or social, will be significant in terms of the course of the COVID-19 pandemic.
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    Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity
    (Taylor & Francis Ltd, 2020) Aşçı, Halil; Özmen, Özlem; Erzurumlu, Yalçın; Savaş, Hasan Basri; Temel, Esra Nurlu; İçten, Pelin; Hasseyid, N.
    We investigated the antioxidant, anti-inflammatory and anti-apoptotic effects of pregabalin (PREG) on lipopolysaccharide (LPS) induced sepsis related cardiotoxicity via NF-k beta pathways. We used 24 female Wistar albino rats divided into three groups: control, LPS treated and LPS + PREG treated. Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), tumor necrosis factor alpha (TNF-alpha), nuclear factor kappa beta (NF-k beta)/p65, p-NF-k beta/p65, caspase-3 (Cas-3) and cleaved Cas-3 were measured in cardiac tissues and creatine kinase MB (CKMB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) levels were measured in blood samples. Also, Cas-3, granulocyte-colony stimulating factors (G-CSF), interleukin-6 (IL-6), serum amyloid A (SAA) and inducible nitric oxide synthase (iNOS) were measured immunohistochemically in heart and aorta tissue. In the LPS group; the levels of CKMB, AST, LDH, TOS, OSI increased and TAS decreased. TNF-alpha, p-NF-k beta/p65 and Cas-3 protein levels also increased in the LPS group. Immunohistochemical evaluation of the heart and aorta revealed a significant increase in the levels of Cas-3, G-CSF, SAA, IL-6 and iNOS in the LPS group. PREG treatment restored all measurements to near normal. LPS induced cardiovascular toxicity was due to inflammation, oxidative stress and apoptosis. PREG ameliorated the damage by inhibition of NF-k beta phosphorylation.
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    Bir geleneksel ve tamamlayıcı tıp uygulama merkezinde yaş kupa terapisi ve Hirudoterapi yapılan hastaların laboratuvar sonuçlarının ve tansiyon ölçümlerindeki değişimin incelenmesi
    (2020) Sarıkan, İsmail; Savaş, Hasan Basri
    Amaç: Geleneksel ve tamamlayıcı tıp (GETAT) uygulamaları, dünya genelinde yaygın olarak yapılmaktadır. Bu çalışmada, ALKÜ Alanya Eğitim ve Araştırma Hastanesi Geleneksel ve Tamamlayıcı Tıp (GETAT) Uygulama Merkezine 30 Mayıs 2018-10 Ocak 2019 tarihleri arasında başvuran kişilerin sayısı, başvuru nedenleri ve başvuruların uygulanan geleneksel yönteme göre sınıflandırılması, uygulama öncesi istenen tetkiklerin geriye dönük olarak değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: ALKÜ Alanya Eğitim ve Araştırma Hastanesi GETAT Uygulama Merkezine ilk seans başvurusu olan 83 kadın, 42 erkek, toplam 125 gönüllü oluru alınarak çalışmaya dâhil edilmiştir. 89 hastaya hacamat (yaş kupa) ve 36 hastaya sülük tedavisi (hirudoterapi) uygulanmıştır. İstatistiksel analiz yapılmıştır. Sonuçlar; sayı, yüzde frekans, ortalama±standart sapma olarak verilmiştir. Kategorik yapıdaki özellikler bakımından grupların karşılaştırılmasında ki-kare testi kullanılmış ve p<0,05 anlamlı kabul edilmiştir. Bulgular: Toplam 125 hastanın ortalama±standart sapma olarak beden kitle indeksi (BKİ) değeri; 29,47±5,69, yaş değeri; 48,90±14,29, meslek dağılımı; ev hanımı 62 (%49) kişi, çiftçi 20 (%16) kişi, işçi 10 (%8) kişi (%8), memur 10 (%8) kişi, diğer (emekli, öğrenci vb.) 23 (%18) kişi olarak bulunmuştur. Açlık kan glukoz değeri 105±43 mg/dL olarak bulunmuştur. Sonuç: Hirudoterapi ve yaş kupa terapisi için başvuran 125 hastanın BKİ açısından fazla kilolu aralığının üst sınırında ve obezite sınırına çok yakın durumda oldukları görülmektedir. Açlık kan glukoz değerleri, normal aralık olarak belirlenen 70-100 mg/dL aralığının üstündedir. Bu sebeplerle GETAT başvurusu yapan hastaların insülin direnci açısından değerlendirilmesi ve insülin direnci varsa uygun tedavi ile tip 2 diyabetes mellitus oluşumunun önlenmesi için çalışılması uygun olacaktır.
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    Bir Tıp Fakültesi Son Sınıf Öğrencilerinin Sağlıklı Beslenme ve Helal Gıdaya İlişkin Bilgi ve Tutumları
    (Alanya Alaaddin Keykubat Üniversitesi, 2017) Yürekli, Mustafa Volkan; Başaran, Özgür; Savaş, Hasan Basri; Kişioğlu, Ahmet Nesimi
    Amaç: Bu çalışmada, tıp fakültesi son sınıf öğrencilerinin helal ve sağlıklıgıda ile alakalı bilgi, tutum, davranış ve algılarının ölçülmesi amaçlandı.Yöntemler: Süleyman Demirel Üniversitesi Tıp Fakültesi sonsınıfında okuyan, 59 erkek, 51 kadın olmak üzere, toplam 110 öğrenciye gözlemaltında anket uygulandı. Katılımcıların gönüllü olurları alındı. Gelirdurumları, ebeveyn meslekleri, cinsiyetleri ile helal ve sağlıklı beslenmearasındaki ilişki anket aracılığıyla sorgulandı.Bulgular: Katılımcıların % 53,6’sı erkek, % 46,4’ü kadındır. Ürün alırken helalsertifikasına dikkat edenlerin oranı % 33,6’dır. Lokantalarda helal sertifikasıarayanların oranı % 25,5’dir. Helal gıda ile ilgili eğitim isteyenlerin oranı %47,3’dür. Helal gıda ile alakalı bilgilerin en sık öğrenildiği kaynaktelevizyon ve internettir. Öğrencilerin sadece % 43,6’sı helal sertifikası olangıdaların sağlıklı olacağını belirtmiştir. Katılımcıların % 25,5’i helalsertifikası alan ürünlerin denetiminin yeterli olduğunu düşünmektedir. Helalsertifikası veren kuruluşların adil davrandığını ifade edenlerin oranı %18,2’dir. Helal sertifikası hakkında bilgi düzeyini iyi olarak ifade edenlerinoranı; % 18,1’dir.Sonuç: Tıp fakültesi öğrencilerinin helal gıda ile sağlıklı beslenme arasındayeterli düzeyde yüksek bir ilişki görmediği, helal gıda ve sertifika hakkındayeterli bilgiye sahip olmadığı, helal gıda belgelerine ve belgeleri verenkuruluşlara yeterince güvenmediği ve gıda seçiminde çoğunluğun helal gıdasertifikasına dikkat etmediği görüldü.
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    Calorie restriction protects against apoptosis, mitochondrial oxidative stress and increased calcium signaling through inhibition of TRPV1 channel in the hippocampus and dorsal root ganglion of rats
    (Springer/Plenum Publishers, 2018) Gültekin, Fatih; Nazıroğlu, Mustafa; Savaş, Hasan Basri; Çiğ, Bilal
    The TRPV1 channel is activated in neurons by capsaicin, oxidative stress, acidic pH and heat factors, and these factors are attenuated by the antioxidant role of calorie restriction (CR). Hence, we investigated the hypothesis that the antioxidant roles of CR and food frequency (FF) may modulate TRPV1 activity and apoptosis through inhibition of mitochondrial oxidative stress in hippocampal (HIPPON) and dorsal root ganglion neurons (DRGN). We investigated the contribution of FF and CR to neuronal injury and apoptosis through inhibition of TRPV1 in rats. We assigned rats to control, FF and FF + CR groups. A fixed amount of food ad libitum was supplemented to the control and FF groups for 20 weeks, respectively. FF + CR group were fed the same amount of food as the control group but with 20% less calories during the same period. In major results, TRPV1 currents, intracellular Ca2+ levels, apoptosis, reactive oxygen species, mitochondrial depolarization, PARP-1 expression, caspase 3 and 9 activity and expression values were found to be increased in the HIPPON and DRGN following FF treatment, and these effects were decreased following FF + CR treatment. The FF-induced decrease in cell viability of HIPPO and DRGN, and vitamin E concentration of brain, glutathione peroxidase, vitamin A, and beta-carotene values of the HIPPO, DRGN, plasma, liver and kidney were increased by FF + DR treatment, although lipid peroxidation levels in the same samples were decreased. In conclusion, CR reduces FF-induced increase of oxidative stress, apoptosis and Ca2+ entry through TRPV1 in the HIPPON and DRGN. Our findings may be relevant to the etiology and treatment of obesity following CR treatment.
  • Küçük Resim
    Öğe
    Cilostazol induces angiogenesis and regulates oxidative stress in adose-dependent manner: A chorioallantoic membrane study
    (2021) Etli, Mutlu; Karahan, Oğuz; Akkaya, Özgür; Savaş, Hasan Basri
    Background: In this study, we aimed to investigate the effects ofcilostazol on angiogenesis and oxidative stress using the chorioallantoicmembrane model. Methods: In this experimental study, the Ross 308 chick embryos wereused. The negative control group (n=10) received no intervention. Thepositive control group (n=10) consisted of eggs treated with epidermalgrowth factor for inducing angiogenesis. Three cilostazol groups weredesigned with 10-7 (n=10), 10-6 (n=10), and 10-5 (n=10) M concentrations.Each egg was punctured on the sixth day of incubation, and drugpellets were introduced to the positive control and drug groups at theprespecified doses. Vascular development was evaluated on the eighthday of application. The total oxidant status, total antioxidant capacity,and oxidative stress index levels were determined from albumen liquidsobtained with a syringe before and after drug application. Results: Lower oxidative stress index levels were obtained fromthe positive control and cilostazol groups compared to the negativecontrol albumens (p=0.001). The increments in vascular junctions andnewly developed vascular nodules were evaluated in drug-free anddrug-applied chorioallantoic membranes. The highest activity wasobtained in the 10-7 M concentration cilostazol group. An increasedangiogenic activity was detected in all drug groups in each concentrationcompared to the negative control group (p=0.001). Angiogenic activitywas similar in all the cilostazol-treated groups (p=0.43).Conclusion: Cilostazol has a positive stimulant effect on angiogenesisand it seems to suppress oxidative stress during embryonic growth.Cilostazol exerts these effects significantly and similarly at differentdoses.
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    Circulation levels of acute phase proteins pentraxin 3 and serum amyloid a in atherosclerosis have correlations with periodontal inflamed surface area
    (Univ Sao Paulo Fac Odontologia Bauru, 2018) Temelli, Başak; Yetkin Ay, Zuhal; Savaş, Hasan Basri; Aksoy, Fatih; Kumbul Doğuç, Duygu; Uskun, Ersin; Varol, Ercan
    Objectives: One of the plausible mechanisms in the relationship between periodontitis and coronary artery disease (CAD) is the systemic inflammatory burden comprised of circulating cytokines/mediators related to periodontitis. This study aims to test the hypothesis that periodontal inflamed surface area (PISA) is correlated with higher circulating levels of acute phase reactants (APR) and pro-inflammatory cytokines/mediators and lower anti-inflammatory cytokines/mediators in CAD patients. Material and Methods: Patients aged from 30 to 75 years who underwent coronary angiography with CAD suspicion were included. Clinical periodontal parameters (probing depth - PD, clinical attachment loss, and bleeding on probing - BOP) were previously recorded and participants were divided into four groups after coronary angiography: Group 1: CAD (+) with periodontitis (n=20); Group 2: CAD (+) without periodontitis (n=20); Group 3: CAD (-) with periodontitis (n=21); Group 4: CAD (-) without periodontitis (n = 16). Serum interleukin (IL) -1, -6, -10, tumor necrosis factor (TNF)-a, serum amyloid A (SAA), pentraxin (PTX) 3, and high-sensitivity C-reactive protein (hs-CRP) levels were measured with ELISA. Results: Groups 1 and 3 showed periodontal parameter values higher than Groups 2 and 4 (p<0.0125). None of the investigated serum parameters were statistically significantly different between the study groups (p>0.0125). In CAD (-) groups (Groups 3 and 4), PISA has shown positive correlations with PTX3 and SAA (p<0.05). Age was found to predict CAD significantly according to the results of the multivariate regression analysis (Odds Ratio: 1.17; 95% Confidence Interval: 1.08-1.27; p<0.001). Conclusions: Although age was found to predict CAD significantly, the positive correlations between PISA and APR in CAD (-) groups deserve further attention, which might depend on the higher PISA values of periodontitis patients. In further studies conducted in a larger population, the stratification of age groups would provide us more accurate results.
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    Effects of nutrition style on neuro-behavior
    (Allied Acad, 2017) Savaş, Hasan Basri; Gültekin, Fatih; Doğuç, Duygu Kumbul
    Objective: The effects of meal frequency and caloric restriction on spatial memory based on hippocampus in rats were examined in our study. Materials and methods: As a result of a four-weeks pilot study amount of food and meal times was determined with 9 rats. In the main study, Wistar albino, 12 weeks, male, 24 rats were divided into three groups as; Ad libitum control (AL) (n=8), two meals fed group (TM) (n=8), two meals fed and 20% calorie restriction of group (TM-CR) (n=8). All rats were kept individually in cages. According to the results of a pilot study in main study; 20 g/day; 10 g for the morning and evening meals were given to TM group, 16 g/day; for the morning and evening meals were given feed in the form of 8 grams to TM-CR group. At the end of the experiment, the hippocampus-based spatial memory training and testing was performed in the Morris Water Maze (MWM) and the experiment was terminated. Results: There was no significant difference between groups about learning speed and memory assessment by WMT (p>0.05). Conclusions: Thus, it was shown that TM and the TM-CR groups have no negative effect in terms of learning and memory. TM and the TM-CR can be presented as a healthy nutritional diet taking into metabolic benefits and no negative impact on learning.
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    Enzymatic behavior of laccase following interaction with gamma-CD and immobilization into PCL nanofibers
    (Academic Press Inc Elsevier Science, 2017) Canbolat, Mehmet Fatih; Savaş, Hasan Basri; Gültekin, Fatih
    This study examines the effects of CD use on enzymatic activity, following enzyme immobilization into nanofibers. There is almost no research available on the change in enzyme activity following interaction with cyclodextrin and electrospun nanofiber mats together. Laccase enzyme was immobilized into nanofibrous structures by various techniques, with and without gamma-CD addition, and the enzymatic activity of the laccase was analyzed. SEM, XRD, and FTIR analyses were used for the characterization of the resulting structures. Our results showed that cyclodextrin use has a positive effect on the enzyme's activity, and increases its stability. The enzymes treated by cyclodextrin showed activation after complex formation trials, and no activation loss or enzyme denaturation was detected. Our conclusions were supported by the enzyme activity test results, which also showed that immobilization by encapsulation methods gave better activity results than layering methods. Another important finding concerned the laccase's stable characteristics that helped to maintain its enzyme activation after the freeze drying process. Among all test groups, the best activity result was recorded by laccase-gamma-CD complex encapsulated PCL nanofibers with 96.48 U/mg. (C) 2017 Elsevier Inc. All rights reserved.
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    Food additives and microbiota
    (2020) Gültekin, Fatih; Öner, Manolya Eser; Savaş, Hasan Basri; Doğan, Bora
    The use of food additives in food production is inevitable in this modern world. Although only a safe amount of food additives is approved, their safety has always been questioned. To our knowledge, the effects of food additives on microbiota have not been investigated in a detailed manner in the literature so far. In this review, the effects of artificial sweeteners, sugar alcohols, emulsifiers, food colorants, flavor enhancers, thickeners, anticaking agents, and preservatives on microbiota were reviewed. Even though most of the results illustrated negative outcomes, few of them showed positive effects of food additives on the microbiota. Although it is difficult to obtain exact results due to differences in experimental animals and models, said the findings suggest that nonnutritive synthetic sweeteners may lead to glucose intolerance by affecting microbiota and a part of sugar alcohols show similar effects like probiotics.
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    Gebelerde ikinci trimester tarama testinde biyokimyasal belirteçler ile gebelik komplikasyonları arasındaki ilişki
    (Alanya Alaaddin Keykubat Üniversitesi, 2017) Savaş, Hasan Basri; Köse, Seyit Ali; Güler, Mesut; Gültekin, Fatih
    Amaç: Downsendromu, neural tube defects (NTDs),  vetrizomi 18 riskinin ve ayrıca AFP,uE3 ve beta hCGseviyelerinin her biri ile doğum öncesi maternal veya fetal komplikasyonlarınve doğum sonrası kromozomal anomalilerin, NTD,yüksek veya düşük ağırlıklı doğumların ortaya çıkması ile ilişkisini araştırmayıamaçladık. Yöntem:Kliniğimizde2010-2012 yılları arasında üçlü tarama testi yapılmış olan 82 hastada gebeliksırasında ortaya çıkan komplikasyonlar retrospektif olarak incelenmiş vegebelik sonrası kromozomal anomali, NTD, yüksek ve düşük doğum ağırlıklı doğumolma durumu ise hasta dosyalarından ve telefonla görüşülerek öğrenilmiştir.AFP, uE3 ve ?hCG seviyeleri Beckman Coulter UniCelDxI 800 cihazında,kemilüminesans yöntemiyle ölçülerek MoM değerleri hesaplanmıştır. Bulgular:Çalışmagrubundaki gebelerin yaş ortalaması 27,7’dir. 8 hastada Downsendromu riski, 10hastada NTD riski, 3 hastada trizomi 18 riski 1/250’nin üzerinde tespitedilmiştir. Gebelik sırasında preeklampsisi olan hasta sayısı 2,gestasyoneldiyabetesmellitusu (DM) olan hasta sayısı ise 8 idi. Düşük doğumağırlıklı doğan bebek sayısı 13 iken yüksek doğum ağırlıklı doğan bebek sayısı11’dir. Sonuç: Yanlışpozitifliklerin daha doğru belirlenmesi için olgu sayısının daha fazla olduğuçalışmalar yapılması ve tarama testlerinin güvenirliklerinin artırılması içingelişmiş klinik araştırmalar yapılması önerilir.
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    Genetiği değiştirilmiş gıdalar ve insan sağlığına etkileri
    (Alanya Alaaddin Keykubat Üniversitesi, 2017) Çatalbaş, Tahir; Savaş, Hasan Basri; Gültekin, Fatih
    Organizmalarıngenetik yapısının değiştirildiği genetik mühendisliği konusu, bilimselplatformlarda hâlâ tartışılmaktadır. Genetiği değiştirilmiş organizmalarıdestekleyen gruplar, bu teknolojinin besin kalitesinin ve sağlığa yönelikfaydalarının artırılmasında, meyve ve sebzelerin raf ömürlerinin veorganoleptik kalitelerinin iyileştirilmesinde, bitkisel ve hayvansal ürünveriminin artırılmasında, yenilebilir aşı ve ilaç üretiminde, insanhastalıklarının tedavisi ve organ nakli için kullanılmasında ve çevresel olarakbirçok faydaları olacağı görüşündedirler. Diğer yandan bu organizmalarıeleştirenlere göre ise besin kalitesindeki değişiklik, gıda güvenliği, alerjikreaksiyonlar ve bunların toksik etkileri ile ilgili önemli riskler olabilir. Bugrup aynı zamanda genetiği değiştirilmiş ürünlerin etiketlenmesi, çevreselsorunlar ile dini, kültürel ve etik sorunlar gibi meselelerinolduğunu/olacağını düşünmektedirler. Gen teknolojisinin oldukça yeni olması veçok hızlı gelişmesi nedeniyle ileri sürülen bütün görüşleri kesin olarakispatlayacak kadar yeterli bilimsel veri bulunmamaktadır. Zaman içindekigözlemler ve araştırmalar bu alana daha iyi ışık tutacaktır.
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    Helal gıda belgelendirmesinde biyokimya laboratuvarının rolü
    (Alanya Alaaddin Keykubat Üniversitesi, 2017) Savaş, Hasan Basri; Çatalbaş, Tahir; Gültekin, Fatih
    ÖZET “Helal” sözcüğü Türk Dili’nde: “Dinikurallara aykırı olmayan” olarak tanımlanır. “Helal Gıda” kavramı, “İslâmîkurallar doğrultusunda izin verilen gıda” anlamında kullanılmaktadır. Helalgıda sertifikaları; sertifika veren bir kuruluşun ürünün tüketiciye ulaşankadar tüm üretim aşamalarında helal kurallarına uygun üretildiğini belgeler.Dünya genelinde helal gıda pazarı oldukça büyük bir ekonomik hacme sahiptir.Helal gıda sertifikalarının kabul görmesi için güvenilir laboratuvarölçümlerine ihtiyaç duyulmaktadır. Bunun için de helal gıda alanındaihtisaslaşmış biyokimya laboratuvarlarının varlığı çok önemlidir. 
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    Improved catalytic activity by catalase immobilization using -cyclodextrin and electrospun PCL nanofibers
    (Wiley, 2017) Canbolat, M. Fatih; Savaş, Hasan Basri; Gültekin, Fatih
    Nanofibrous structures are promising for biocatalyst immobilization due to their large surface area which facilitates the enzyme attachment, stability, ease of separation, and fine porous structure. There is limited research available on the change in enzyme activity following interaction with cyclodextrin. In this study, catalase enzyme was immobilized into nanofibrous structures by various techniques, with and without -CD addition, and the enzymatic activity of catalase was evaluated. In addition, catalase--CD complex containing PEO polymer solution was electrospun in between PCL nanofibrous layers as a newly developed technique. The enzyme immobilized nanofibrous structures were characterized by SEM, XRD, and FT-IR analysis methods. Among all the activity tests, best enzyme activity was recorded with catalase--CD physical mixture encapsulated PCL nanofibrous layers. Moreover, the test results indicated that the use of cyclodextrin in immobilization process considerably improves the catalytic activity of the enzyme. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44404.
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    İnsülin direnci ve klinik önemi
    (2017) Gültekin, Fatih; Savaş, Hasan Basri
    İnsülin metabolizmanın düzenleyici temel hormonlarından biridir. İnsülinin keşfi için uzun asırlar boyu süren araştırmalar mevcuttur. Bilim adamlarını insülini araştırmaya iten sebep insülin direncinin ortaya çıkardığı hastalıklardır. İnsülin direnci, karmaşık bir patogeneze sahiptir. İnsülin direncinin patogenezi halen tam olarak aydınlatılamamıştır ve araştırılmaktadır. Obezite ile insülin direnci arasında çok güçlü bir ilişki bulunmaktadır. İnsülin direncinin en sık sebebi obezitedir. İnsülin direnci ortaya çıktığında hemen klinik belirti vermeyebilir. Subklinik dönemde diyet düzenlenmesi ve egzersizi içeren yaşam tarzı değişiklikleri ve kilo verilmesi ile insülin direnci gerileyebilir. İnsülin direncine müdahale edilmediğinde, uzun dönemde birçok hastalığa sebep olabilir. Kardiyovasküler hastalık riski artar. Endotel fonksiyonu bozulur. Tip 2 Diyabetes Mellitus gelişir. Metabolik sendrom sıklığı gittikçe artmaktadır. Metabolik sendromun en önemli bileşeni insülin direncidir. İnsülin direncinin ölçümü için altın standart olan metot, öglisemik hiperinsülinemik klemp metodudur. Bu metodun zahmetli ve pahalı olması sebebiyle HOMA (Homeostasis Model Assessment) metodu kullanılmaktadır. HOMA formülü açlık serum glikozu ve açlık serum insülini değerleri ile hesaplamaya dayanır. İnsülin direnci varlığında, yaşam tarzı değişiklikleri ile insülin direncini geriletme hedeflenir. Şayet geriletilemeyecek duruma gelmiş ve klinik problemler ortaya çıkmışsa hastalığa uygun ilaç tedavisi verilebilir. İnsülin direnci temelinde ortaya çıkan hastalıkların medikal tedavisine ek olarak yaşam tarzı değişiklikleri ilaçların etkinliğini arttırmaktadır. İnsülin direncine karşı yapılacak yaşam tarzı değişiklikleri, kilo verilmesi, diyet düzenlenmesi ve düzenli egzersiz olarak sayılabilir.
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    Melatonin protects the heart and endothelium against high fructose corn syrup consumption-induced cardiovascular toxicity via SIRT-1 signaling
    (Sage Publications Ltd, 2019) Savran, Mehtap; Aşçı, Halil; Özmen, Özlem; Erzurumlu, Yalçın; Savaş, Hasan Basri; Sönmez, Yasin; Şahin, Yasemin
    High fructose corn syrup (HFCS) has been shown to cause cardiovascular toxicity via oxidative stress and inflammation. The aim of this study is to demonstrate the protective effects of melatonin (MLT) against HFCS-induced endothelial and cardiac dysfunction via oxidative stress and inflammation. Thirty-two Sprague Dawley male rats were distributed into three groups as control, HFCS, and HFCS + MLT. HFCS form F55 was prepared as 20% fructose syrup solution and given to the rats through drinking water for 10 weeks, and MLT administrated 10 mg/kg/day orally for last 6 weeks in addition to F55. After decapitation, blood and half of the heart samples were collected for biochemical analysis and other half of the tissues for histopathological and immunohistochemical analysis. Aspartate transaminase, creatine kinase MB, lactate dehydrogenase, total oxidant status and oxidative stress index, and caspase-3 levels increased and total antioxidant status levels decreased significantly in HFCS group. MLT treatment reversed all these parameters. Histopathologically, hyperemia, endothelial cell damage and increased levels of angiogenin, C-reactive protein, inducible nitric oxide synthase, myeloperoxidase and decreased sirtuin-1 (SIRT-1) expressions were observed in HFCS group. MLT ameliorated all these changes. MLT has an anti-inflammatory, antioxidant, antiapoptotic effects on HFCS-induced cardiovascular toxicity through enhancing the expression of SIRT-1.
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    Occupational Health Assessment of Permanent University Employees in Terms of Clinical Biochemical Data
    (2021) Savaş, Hasan Basri
    Aim: Occupational health and safety involves important measures, trainings, and health screenings as defined by law. The existing literature on occupational medicine practices and related clinical laboratory data has considerable room for expansion. The aim of this study is to contribute to the evaluation of workplace medicine practices with original laboratory data. Material and Method: The clinical laboratory data of the workers from the Alanya Alaaddin Keykubat University, Turkey, were analyzed retrospectively. Biochemical test results of a total of 104 permanent worker, 43 women and 61 men, were compared. Statistical analysis results were evaluated and p<0.05 was accepted as the limit of significance. Results: In the statistical analysis based on sex, AST (Aspartate Aminotransferase), ALT (Alanine aminotransferase), RBC (Red blood cell), HGB (Hemoglobin), HCT (Hematocrit), MCH (Mean Corpuscular Hemoglobin; Average Cell Hemoglobin), MCV (mean erythrocyte volume), MCHC (Average Cell Hemoglobin Concentration), MONO (Monocyte) and MONO% values are higher in male workers, whereas PLT (platelet), PCT (Percent ratio of platelet cells to other cells), RDW-CV (Erythrocyte distribution width-coefficient variation-coefficient of variation) and RDW-SD (Erythrocyte distribution width-standard deviation) values are higher in female workers (p<0.05). Discussion: In this study, the young age of the participating workers prevented laboratory results to go off the reference range. Values that differ between female and male gender groups are in line with expected differences based on gender. Including the fasting blood glucose values in routine clinical laboratory tests requested within the scope of workplace medicine may be useful in the early diagnosis and prevention of increasingly frequent insulin resistance, obesity, and type 2 diabetes mellitus.
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    Oxidant-Antioxidant Balance and Trace Elements in Children with Functional Dyspepsia
    (2021) Savaş, Hasan Basri; Sayar, Ersin
    Objective: Functional dyspepsia (FD) is the most common functional abdominal pain disorder among children and adolescents according to the Rome IV criteria. The aim of the study was to determine the possible effect of the equilibrium of oxidant and antioxidant and trace elements in pediatric FD. Material and Methods: The patient group consisted of 23 children who were diagnosed with FD according to the Rome IV Questionnaire on Pediatric Gastrointestinal Disorders. The control group consisted of 23 children aged 11-17 who did not meet the Rome IV criteria and had no chronic disease. Measurements of zinc, copper levels and recently developed new generation oxidant-antioxidant balance markers were performed spectrophotometrically using a commercial kit. Routinely measured hemogram and vitamin B12 and vitamin D test results were obtained from the patient files retrospectively. Results: In comparison of the control group, the pediatric FD group had significantly higher levels of oxidized thiol, ischemia-modified albumin, neutrophil and neutrophil/lymphocyte, a significantly lower lymphocyte count and reduced thiol ratio, and significantly lower vitamin D, native thiol, and copper levels. Conclusion: FD is potentially related to inflammation, serum copper content, and oxidative stress. However, the relationship between FD and inflammation, copper levels, and oxidative stress has not been adequately investigated in children. As a conclusion of this clinical trial, thiol balance, ischemia modified albumin, neutrophil/lymphocyte ratio, vitamin D and copper content will likely be essential for diagnosis and follow-up in pediatric FD
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    Parao[onase 1 Activities in First Trimester Miscarriages
    (2021) Savaş, Hasan Basri; Erdem, Dilek
    Aim: Miscarriages are becoming increasingly important problem nowadays. First trimester pregnancy loss frequency in women of reproductive age is increasing. In this study, it is aimed to show the possible relationship between first trimester pregnancy losses and PON1 and ARY activities. Material and Method: 25 pregnant healthy women (Group 1) and 25 women with first trimester miscarriage (Group 2) in 6-14 gestational weeks were included in this study. Paraoxonase 1 and arylesterase activities of the sera samples were investigated using fully automated colorimetric methods. ANOVA test was used to compare the groups. Results: PON1 and ARY activities are statistically significantly lower in pregnant women with first trimester miscarriage compared to healthy pregnant women in first trimester analyzed by Anova test (p<0.05). Conclusion: The results of this study showed that paraoxonase 1 and arylesterase antioxidant enzyme activities can be evaluated as significant predictive clinical laboratory parameters in first trimester pregnancy losses. If this study is confirmed with larger patient groups, our research results will gain more value.
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    Paraoxonase 1 activity as a new biochemical marker in the diagnosis of peripheral arterial disease
    (2021) Savaş, Hasan Basri; Etli, Mustafa
    Aim: Peripheral artery disease (PAD) is an atherosclerotic disease. It is seen in older ages. It causes cardiovascular morbidity and mortality. PAD may progress without any symptoms. Despite its high frequency, there is no laboratory parameter that directly indicates peripheral arterial disease in routine biochemical tests. The relationship between oxidative stress increase and PAD is known. In this study, it is aimed to show the possible usage of the activities of the antioxidant enzymes paraoxonase 1 and arylesterase as a new marker in the diagnosis of PAD. Material and Methods: A total of 70 individuals, including 35 in the control group and 35 peripheral artery patients, were included in this study. The collected blood serums were separated and stored at -80 °C. Paraoxonase 1 and arylesterase activities were measured using the spectrophotometric method in the serum which was dissolved at room temperature. The results were subjected to statistical analysis. P <0.05 was accepted as the level of significance. Results: In the peripheral arterial disease group, the paraoxonase 1 and arylesterase activities were found to be significantly lower than those in the control group (p <0.05). In peripheral arterial disease, paraoxonase 1 and arylesterase activities were shown to decrease. Conclusion: In peripheral arterial disease, paraoxonase 1 and arylesterase activities were found to decrease significantly. The results of similar studies related to atherosclerosis in the literature were in line with our findings. It would be beneficial to support the results of this study with new studies and evaluate paraoxonase 1 and arylesterase activities in routine biochemistry laboratories for the diagnosis and follow-up of peripheral arterial disease.