Yazar "Ozgur, Aykut" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ X ]
    Öğe
    A Silver Compound with Potential Applications as an Anticancer, Antibacterial, Antifungal, and Anti-Alzheimer's Agent
    (Wiley-V C H Verlag Gmbh, 2023) Korkmaz, Nesrin; Kisa, Dursun; Kaplan, Ozlem; Tosun, Nazan Goksen; Ozgur, Aykut; Imamoglu, Rizvan; Karadag, Ahmet
    This study investigated the biological activity of the silver coordination compound K-22. The IC50 values of K-22 on cancer cell lines range from 0.797 mu g/mL to 3.426 mu g/mL, indicating that K-22 might preferably inhibit A549, Saos-2, MCF-7, and HT-29 cell proliferation and thus have better therapeutic activity. Furthermore, K-22 stimulated apoptosis via up-regulation of the mRNA and protein expression level of Bax/Bcl-2 ratio in A549, Saos-2, MCF-7, and HT-29. K-22 exhibited antimicrobial activity against S. aureus, E. faecalis, K. pneumonia, P. aeruginosa, C. utilis, and C. albicans. Experimental results show that the compound has inhibitory potential with an IC50 value of 178.10 mu M for the BChE (butyrylcholinesterase) enzyme, which has a vital role in the progression of Alzheimer's disease. As a result, compound K-22 exhibits a strong potential for medical use due to its anticancer, antibacterial, antifungal, and anti-Alzheimer properties.
  • [ X ]
    Öğe
    Efficacy of the greenly synthesized silver, copper, and nickel nanoparticles using Allium tuncelianum extract against Acanthamoeba castellanii
    (Elsevier, 2023) Aykur, Mehmet; Tosun, Nazan Goksen; Kaplan, Ozlem; Ozgur, Aykut
    Acanthamoeba is a common protozoan in many environments, leading to infection in humans and animals. Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE) are caused by Acanthamoeba. AK is an infection in the eye that can lead to vision loss and does not have a fully effective treatment. Therefore, there is an urgent need to develop new therapies against Acanthamoeba for the treatment of AK and GAE. Green nanotechnology synthesis approaches have been recently reported to be more environmentally friendly and effective in antimicrobial, antiviral, antifungal, and antiprotozoal activities. Therefore, they might be a good strategy for developing anti-Acanthamoeba substances. This study aimed to use the microwave-assisted method to prepare AgNPs, CuNPs, and NiNPs using the crude extract of Allium tuncelianum (AT). Moreover, the synthesized AT-AgNPs, AT-CuNPs, and AT-NiNPs were characterized using UV-Visible spectroscopy, DLS, and FTIR tech-niques. The first time anti-amoebic activity of AT-AgNPs, AT-CuNPs, and AT-NiNPs was evaluated against Acanthamoeba castellanii. Anti-amoebic activity as IC50 value of AT-AgNPs, AT-CuNPs, and AT-NiNPs was observed 1556.56 +/- 7.36 mu g/ml, 1826.44 +/- 17.84 mu g/ml, and 2014.23 +/- 7.04 mu g/ml after 24 h, respectively. After 24 h, AT-AgNPs were shown to be superior to other NPs in killing Acanthamoeba trophozoites at a 2000 mu g/ ml concentration. AT-AgNPs' IC50 value was determined to be effective against Acanthamoeba trophozoites at a concentration almost twice as low as PVP-I's IC50 value after 48 h. At doses of 500 mu g/ml, 1000 mu g/ml, and 2000 mu g/ml, the impact of AT-AgNPs on the viability of 50% Acanthamoeba trophozoites was assessed after 48 h. The conclusion of the present study demonstrates the most effective of AT-AgNPs among the nanoparticles when used against the treatment of infections caused by A. castellanii. These agents show the potential to create new, efficient, and secure treatment options.
  • [ X ]
    Öğe
    Evaluation of Anticancer and Antimicrobial Potentials of Tarantula cubensis Venom (Theranekron® D6)
    (2024) Tosun, Nazan Gökşen; Kaplan, Ozlem; Ozgur, Aykut
    Tarantula cubensis venom (Theranekron®D6) is widely used in veterinary medicine as a drug with anti-tumor, wound healing, anti-inflammatory, antiphlogistic properties. The purpose of the study was to explore Theranekron®D6 (TD6)'s antibacterial activity and its impact on apoptotic cell death in human colon and liver cancer cell lines. TD6 showed a dose and time dependent cytotoxic effect at 12h and 24h in HT-29 and HUH-7 cancer cell lines. The IC50 values of TD6 were calculated as 12.18 µg/mL and 25.10 µg/mL in HT-29 and HUH-7 cell lines at 24h, respectively. TD6 induced apoptosis in HT-29 and HUH-7 cell lines. In these cells exposed to TD6, while the BAX/BCL-2 ratio and CASP-3 mRNA level increased, the HSP90 mRNA and protein level decreased. Also, TD6 exhibited antimicrobial activities against Pseudomonas aeruginosa, Enterococcus faecalis, Klebsiella pneumonia, Staphylococcus aureus, Candida albicans and Candida utilis. Obtained results demonstrated that TD6 has great potentials as alternative therapeutic for cancer and infectious diseases as apoptosis inducer and antimicrobial agent.