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    Protective effect of Lumbricus rubellus Hoffmeister extract in experimental renal ischemia/reperfusion injury in the nephrectomy rats
    (Natl Inst Science Communication-Niscair, 2023) Oz, Semih; Yildiz, Fatma; Kabay, Sahin; Senturk, Hakan; Donmez, Dilek Burukoglu; Ozbayer, Cansu; Ustuner, Mehmet Cengiz
    Acute kidney injury (AKI), an immediate loss of renal function, leads to high mortality, and ischemia-reperfusion (I/R) injury is considered as one of the main causes of AKI. Inflammation and oxidative stress are known to play an important role in AKI. On the other hand, the earthworm extract, used in traditional medicine, is known to possess various biological and pharmacological activities viz. antiapoptotic, anticoagulative, fibrinolytic, anti-inflammatory, antioxidative stress, peripheral nerve regeneration, bone regeneration and wound healing. Hence, in this study, we investigated the protective effect of the earthworm Lumbricus rubellus Hoffmeister extract (LE) after nephrectomy, against oxidative stress occurring during renal ischemia/reperfusion (I/R) injury. A total of 10-12 weeks old Sprague Dawley male rats were divided into five groups (n=8). Group I (control), Group II (I/R), Group III (I/R + 10 mg/kg LE), Group IV (I/R + 20 mg/kg LE) and Group V (I/R + 40 mg/kg LE). All rats except in Gr. I were applied ischemia for 45 min and reperfusion for 24 h. At the end of the experiment, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) enzyme activities and malondialdehyde (MDA) levels were evaluated. In addition, kidney tissues were evaluated histologically. In results, the MDA and GPx level of the I/R group were found to be significantly higher than the control and LE groups. SOD activity of the control group did not differ when compared to LE groups and CAT levels were not significantly different between all groups. In addition, in Gr. III-V we observed nearly normal renal cortex and renal tubules. The present study, thus demonstrates that the extract of L. rubellus prevents renal I/R injury and induced biochemical and histological changes in the renal tissues in rats.
  • [ X ]
    Öğe
    Protective Effects of Lumbricus Extract on the Antioxidant System and Liver in an Experimentally Created Liver Ischemia Reperfusion Injury Model in Rats
    (Pleiades Publishing Inc, 2023) Oz, Semih; Yildiz, Fatma; Senturk, Hakan; Ustuner, Mehmet Cengiz; Donmez, Dilek Burukoglu; Kus, Gokhan; Ozbayer, Cansu
    In this experimental study, we investigated the effects of Lumbricus extract on biochemical and histological markers of liver IR injury model in rats. A total of 10-12-weeks old, 250-300 g Sprague Dawley male rats were divided into groups of five (n = 7). Group I (Sham), Group II (IR), Group III (IR + 10 mg/kg Lumbricus extract), Group IV (IR + 20 mg/kg Lumbricus extract), and Group V (IR + 40 mg/kg Lumbricus extract) were marked, respectively. Lumbricus extract was dissolved with saline and administered orally. All rats except in Group I were applied ischemia for 45 minutes and reperfusion for 24 hours. The data obtained at the end of the experiment were evaluated by histological and biochemical methods, where we investigated catalase (CAT) superoxide dismutase (SOD) enzyme activity and malondialdehyde (MDA) levels, as well as a histological examination of liver tissue samples. When MDA enzyme activity levels were compared with the sham and Lumbricus extract groups, it was found to be significantly higher than the IR group. SOD and CAT activity enzyme levels were found to be significantly lower. Our findings showed that Lumbricus extract could have a protective effect against experimentally induced IR injury in the liver by increasing antioxidant enzyme levels and decreasing lipid peroxidation and liver injury.