Yazar "Ovey, Ishak Suat" seçeneğine göre listele

Listeleniyor 1 - 5 / 5
  • [ X ]
    Öğe
    Effect of combination treatment of protocatechuic acid with 5-fluorouracil and oxaliplatin on colon cancer Caco-2 cell line
    (Natl Inst Science Communication-Niscair, 2023) Yildiz, Fatma; Eciroglu, Hamiyet; Ovey, Ishak Suat; Avnioglu, Seda
    Among the most common antitumor drugs used in the treatment of colon cancer are 5-fluorouracil and oxaliplatin (5-FU and OXA). However, both these drugs have many side effects, and hence there is a need for new treatment\approach to reduce the side effects aas well as drug concentration. In this context, here, we investigated the effect of addition of protocatechuic acid (PCA) onto either monotherapies or combination therapies of 5-FU and OXA on the human colon cancer (Caco-2) cell line. In addition, we did evaluate the synergistic effect of PCA with 5-FU and OXA. Further, we determined the suppressive effects of different doses of PCA alone or in combination with 5-FU/OXA on cell proliferation after 24 and 48 hours. We identified a suppressive effect of PCA on cell viability at 48 h starting from the dose of 50 mu M Matrix metalloproteinase-2 (MMP-2) and MMP-9 gene expression levels and apoptotic effects showed significant increases and decreases depending on the dose and time applied in the experimental groups. The highest synergistic activity was seen at 2:1 concentration of 5-FU+ PCA. Our findings indicate the presence of the cytotoxic and apoptotic effects of PCA in Caco-2 cells at 48 h, increasing with a dose-and time-dependent manner.
  • [ X ]
    Öğe
    EFFECT OF MELATONIN AND CISPLATIN ON CERVICAL CANCER CELLS: ROLE OF TRPV1 CHANNELS
    (Publ House Bulgarian Acad Sci, 2025) Ovey, Ishak Suat; Ates, Sezin
    Cisplatin, a widely used treatment for cervical cancer, and melatonin, known for enhancing the apoptotic effects of cisplatin, both play crucial roles in our study. We investigated the combined effects of these two agents on HeLa cervical cancer cells, with focus on the role of TRPV1 channels, which we found to be of significant importance. According to the treatment performed, the cell samples were divided into seven main groups: control, cisplatin, cisplatin + capsazepine (TRPV1 antagonist), melatonin, melatonin + capsazepine, cisplatin + melatonin, and cisplatin + melatonin + capsazepine. Each group was stimulated with capsaicin (TRPV1 agonist) in all analyses. We measured apoptosis, cytosolic calcium, intracellular reactive oxygen species, mitochondrial depolarization, caspase-9, and caspase-3 levels. The combined application of cisplatin and melatonin led to a significant increase in apoptosis levels, which were statistically significantly higher compared to both the control group and the group treated with cisplatin alone (p < 0.001) (Fig. 2). This notable increase underscores the potential of this combined treatment in treating cervical cancer. Our findings strongly suggest that melatonin could be a promising adjunct therapy for cisplatin-induced cervical cancer. By elevating cytosolic Ca2+ levels, promoting apoptosis, and increasing intracellular ROS levels through TRPV1 channels, melatonin indicated potential as an effective
  • [ X ]
    Öğe
    EGb 761 reduces Ca2+ influx and apoptosis after pentylenetetrazole treatment in a neuroblastoma cell line
    (Frontiers Media Sa, 2023) Ovey, Ishak Suat; Ozsimsek, Ahmet; Velioglu, Halil Aziz; Altay, Ozlem; Mardinoglu, Adil; Yulug, Burak
    Background: Transient receptor potential (TRP) channels have been found to have significant implications in neuronal outgrowth, survival, inflammatory neurogenic pain, and various epileptogenic processes. Moreover, there is a growing body of evidence indicating that transient receptor potential (TRP) channels have a significant impact on epilepsy and its drug-resistant subtypes.Objective: We postulated that EGb 761 would modulate TRPA1 channels, thereby exhibiting anti-inflammatory and neuroprotective effects in a neuroblastoma cell line. Our rationale was to investigate the impact of EGb 761 in a controlled model of pentylenetetrazole-induced generalized epilepsy.Methodology: We evaluated the neuroprotective, antioxidant and anti-apoptotic effects of EGb 761 both before and after the pentylenetetrazole application in a neuroblastoma cell line. Specifically, we focused on the effects of EGB 761 on the activity of Transient receptor potential (TRP) channels.Results: EGb 761 applications both before and after the pentylenetetrazole incubation period reduced Ca release and restored apoptosis, ROS changes, mitochondrial depolarization and caspase levels, suggesting a prominent prophylactic and therapeutic effect of EGb 761 in the pentylenetetrazole-induced epileptogenesis process.Conclusion: Our basic mechanistic framework for elucidating the pathophysiological significance of fundamental ion mechanisms in a pentylenetetrazole treated neuroblastoma cell line provided compelling evidence for the favorable efficacy and safety profile of Egb 761 in human-relevant in vitro model of epilepsy. To the best of our knowledge, this is the first study to investigate the combined effects of EGb 761 and pentylenetetrazole on TRP channels and measure their activation level in a relevant model of human epileptic diseases.
  • [ X ]
    Öğe
    Protective and Therapeutic Role of Ginkgo Biloba Extract Through TRPV1 Channels in an in Vitro Alzheimer's Disease Model
    (Turkish Neuropsychiatry Assoc-Turk Noropsikiyatri Dernegi, 2023) Ozsimsek, Ahmet; Ovey, Ishak Suat; Karacay, Ertan; Yulug, Burak
    Introduction: The effect of Ginkgo biloba (GB) on mitochondria dependent TRPV1 ion channels in neuroblastoma cells was investigated by creating an Alzheimer's disease (AD) model.Methods: Okadaic acid was applied on SH-SY5Y cells to create an AD model. After cellular differentiation, the study was organized with the seven main groups, examining the effect of GB on calcium depended TRPV1 channels in neuroblastoma cells AD, has been established in vitro.Results: The higher Ca2+ concentration was detected in the GB+AD, AD and AD+GB groups when compared with the control (p<0.001). The Ca2+ level was lower in GB+AD and AD+GB groups than in the AD group (p<0.001). Also, cytosolic Ca2+ concentration was lower in the GB+AD than in the AD+GB group (p<0.05), the apoptosis and intracellular reactive oxygen species (ROS) values were higher in the GB+AD, AD and AD+GB groups than in the control (p<0.001). The apoptosis and intracellular ROS values were higher in AD group than in the GB+AD and AD+GB group (p<0.001) and the apoptosis level was higher in AD+GB group than GB+AD group (p<0.001) and the mitochondrial depolarization, caspase 3 and caspase 9 levels were higher in the GB+AD, AD and AD+GB groups when compared to the control group (p<0.001). Also, the values were lower in the GB+AD group, AD group and AD+GB groups when compared with the GB+AD+capsazepine group, AD+capsazepine group and AD+GB+capsazepine respectively (p<0.001).Conclusion: These results show us that GB has a protective effect besides its therapeutic effect in Alzheimer's disease via TRPV1 channel.
  • [ X ]
    Öğe
    The use of Hückel and Onsager equation to derive stimulating voltage for a cell
    (Nature Portfolio, 2025) Aydin, Ismail Erkan; Ovey, Ishak Suat; Bektas, Hesna; Aydin, Mehmet
    This article deals with the use of H & uuml;ckel-Onsager equation to determine potential drop in the voltage-dependent ion channels of cell membranes. The necessary parameters/variables were derived from literature to describe how one calculates entry potential at a hypothetical plane in the selectivity filter of ion channels. We start with computation of entry potential for a resting potential of -70 mV as soon as a few voltage-gated Na+ channels open. Calculated corresponding entry potential was - 15.01 mV. A simplified approach linked to this reference value of entry potential was devised for quantifying stimulus voltage to generate an action potential in the excitable cells with different resting potentials. Threshold voltage of stimulus, which is enough strength to depolarize cell membrane to threshold level, varied between 8.58 and 17.16 mV for the resting potentials of -40 and - 80 mV, respectively. The magnitude of difference between resting and threshold potentials was wider in case of higher negative resting voltages. The velocity and number of ions passing through one channel were indirectly computed for an estimated entry potential/stimulus voltage. Their quantities were the power functions of electrical field strengths. This work can lead to improving strategies for early management of status epilepticus in emergency departments.