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  • Küçük Resim
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    Plasma thiol/disulphide homeostasis changes in patients with relapsing-remitting multiple sclerosis
    (2021) Özben, Serkan; Küçüksayan, Ertan; Köseoğlu, Mesrure; Erel, Özcan; Neşelioğlu, Salim; Özben, Tomris
    Background: Multiple sclerosis (MS) is a neuroinflammatory disease and inflammation and oxidative stress play important roles in its pathology. Thiol/disulphide homeostasis (TDH) is a special oxidative stress biomarker that has been found to be affected in several disorders including MS. There is no study demonstrating the effects of attack status of the relapsing-remitting multiple sclerosis (RRMS) patients on TDH levels. Our aim was to determine TDH levels in three different periods of RRMS patients and healthy individuals. Methods: The study was carried out in 29 patients with RRMS without a prior attack in the last twelve months (MS Control), 21 RRMS patients having a clinical acute attack within the last week (MS relapse), 12 of 21 MS relapse patients one month after the onset of attack and following 1000 mg methylprednisolone for 7 days (MS Remission) and 30 age- and sex-matched healthy individuals. TDH status was determined using an automated spectrophotometric analysis method. TDH levels in all patient groups and control subjects were compared with each other. Results: The lowest native thiol, total thiol levels and native thiol/total thiol ratio were found in the MS relapse patients in comparison to the MS control, MS remission groups and healthy controls. In contrast, disulphide levels, disulphide/native thiol and disulphide/total thiol ratios were highest in the MS relapse group compared to the other patient groups and healthy subjects. Conclusion: Our findings indicate that increased oxidative stress in RRMS patients is reflected with decreased native and total thiol and increased disulphide levels. Since the formation of disulphide bonds is reversible, the progression of RRMS involving abnormal TDH may be controlled, converting disulphides to thiols. So, we suggest determining the dynamic TDH status as a novel and special biomarker in the diagnosis and prognosis of the RRMS patients. What’s known • Multiple sclerosis (MS) is a neuroinflammatory disease and inflammation and oxidative stress play important roles in its pathology. • Like other inflammatory diseases, oxidative stress is associated with MS playing an important role in the pathogenesis of MS. What’s new • Our study provides original data on dynamic Thiol/disulphide homeostasis (TDH) status in the relapsing-remitting multiple sclerosis (RRMS) patients at three different periods of the disease and examines whether plasma TDH can be used as a special oxidative stress biomarker in the diagnosis and follow-up of the RRMS patients and their response to the therapy
  • Küçük Resim
    Öğe
    The Effect of Regularly Performed Oderate-Intensity Exercise Program on Thiol/Disulfide Homeostasis, and Ischemia-Modified Albumin
    (2022) Pala, Mukaddes; Altan, Mehmet; Hanikoğlu, Ferahat; Neşelioğlu, Salim; Erel, Özcan; Metin, Gökhan
    Aim: Thiol/disulfide homeostasis is an indicator of oxidative stress and antioxidant capacity. Ischemia-modified albumin (IMA) is an important marker for both oxidative stress and ischemia. We aimed to evaluate the possible effects of regularly performed moderate-intensity exercise on thiol/disulfide homeostasis, and IMA levels. Methods: Sprague Dawley rats were used. The study was composed of an Exercise group (EG, n=9) and Control group (CG, n=6). A 10-weeks swimming exercise was performed. Thiol/disulfide homeostasis measurement method was used in this study. IMA levels were measured by a cobalt-albumin binding method. Results: In the EG, total thiol levels were significantly higher compared to the CG (p<0.01). The disulfide/total thiol ratio was lower in the EG compared to the CG (p<0.01). We observed that there was a slight increase in IMA levels in EG (p=0.18). This increase was not statistically significant. Conclusion: Regularly performed moderate-intensity exercise has increased native and total thiol levels. Increase of thiol levels can prevent oxidative stress. Regularly performed moderate-intensity exercise programs appear to provide favourable effects on oxidative stress.