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    Do irrigation solutions effect bond strength of composite resin to deep margin elevation material? An in-vitro study
    (Bmc, 2025) Mutlu, Seref Nur; Fidancioglu, Yasemin Derya; Ozkan, Hatice Buyukozer; Ulker, Hayriye Esra
    Background The deep margin elevation (DME) technique provides advantages for root canal treatment, but the impact of applied irrigation protocols on bonding for subsequent restorations is a significant concern. The aim of this in vitro study was to test the bond strength of a flowable resin material used in DME restorations after composite restorations were exposed to different irrigation protocols. Methods Standard composite samples (G-aenial (R) Universal Injectable) were divided into nine groups according to irrigation protocols. Untreated composite sample were used as control. The group A were kept in 5.25% sodium hypochlorite (NaOCl) and the group B were kept in 3.5% chlorine dioxide (ClO2) for 30 min. Then, the following treatment protocols were applied to the subgroups, respectively. Group1A/B: 17%EDTA + 5.25%NaOCl + Distilled Water + 2%CHX, Group2A/B: 18%HEDP + 5.25%NaOCl + Distilled Water + 2%CHX, Group3A/B: 17%EDTA + 3.5%ClO2 + Distilled Water + 2%CHX, Group4A/B: 18%HEDP + 3.5%ClO2 + Distilled Water + 2%CHX. After irrigation procedures, samples were washed with distilled water and sandblasted. G-Premio Bond and composite restorations (G-aenial (R) A'CHORD) were applied. The samples were then cut perpendicular to the interface with an IsoMet (R) low speed diamond saw under water. For the microtensile bond strength test, rectangular sticks with an average cross-sectional area of similar to 1 mm(2) will be obtained. The first section with 1-mm thickness was excluded to prevent its possible confounding effect on the results. Three sections were obtained of each sample (n = 15) and tested for microtensile bond strength. The analysis of the data collected in accordance with the purpose of the study was performed with One-way ANOVA (n = 15). For multiple comparisons between groups, it was evaluated with Tukey HSD test. Results Groups A1 and A2, immersed in NaOCl for 30 min, showed statistically significantly lower bond strength compared to Group B3, immersed in ClO2 for 30 min, and the control group (P < 0.05). The use of ClO2 irrigation is recommended due to its positive effects on bond strength. Conclusion Prolonged NaOCl irrigation may adversely affect the bond strength of flowable composites used for deep margin elevation.