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    Öğe
    Effect of silver ion surface coating on antimicrobial and cutting efficiencies of nickel-titanium rotary files
    (Galenos Yayincilik, 2020) Cora, Sabri; Er, Kürşat; Taşdemir, Tamer; Kiraz, Nadir; Becer, Burcu; Felek, Rasih; Er, Hakan
    Objective: Silver ions are well known to have effective antimicrobial activity against many microorganisms. This study aims to evaluate the antimicrobial and cutting efficiencies of nickel-titanium rotary file surface coated with silver ions by using the dip-coating method. Materials and Methods: A pilot study was performed before the endodontic file coating. The prepared coating solutions (silan and silver-complex systems) were applied to stainless steel plates by using the spin-coating method. The antibacterial activity of the coatings was researched against Enterococcus faecalis, which resulted in the selection of the coating solution with a 20% solid ratio and 2% silver content. Subsequently, the files were assigned to three groups based on the coating speed [group 1, 50 mm/min; group 2, 25 mm/min; and group 3, non-coated files (control)]. The cutting efficiency was evaluated by calculating the weight loss of the transparent acrylic blocks. The file surface and section was evaluated using scanning electron microscopy (SEM) and SEM-energy-dispersive X-ray spectroscopy before and after preparation. Data were statistically analysed with one-way analysis of variance and post-hoc Bonferroni test (p<0.05). Results: Significantly more debris was removed from Group 1. The cutting efficiency was not significantly different between groups 2 and 3. Groups 1 and 2 showed distinctive surface irregularities. Conclusion: The application of this antimicrobial surface coating was promising for improving antibacterial effectiveness of nickel-titanium rotary files. In addition, the thin film coating did not cause any negative effects on file cutting efficiency.
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    Öğe
    Enhanced anticancer effect of cetuximab combined with stabilized silver ion solution in EGFR-positive lung cancer cells
    (2019) Erdoğan, Ayşe; Özkan, Aysun; Özkan, Ödül; Manguoğlu, Esra; Kiraz, Nadir
    Background: Cytotoxic, antiproliferative, cell cycle inhibi- tive, oxidative and apoptotic effects of cetuximab [anti- body for epidermal growth factor receptor (EGFR)] alone and together with stabilized silver ion solution (St-Ag) on P-H1299, R-H1299, A-431 and A-549 cells were investigated. Materials and methods: Cytotoxic effects of cetuximab alone and together with St-Ag on cells were determined by Cell Titer-Blue® Cell Viability and Lactate Dehydrogenase Activ- ity tests. Cell cycle distributions and apoptosis were detected by reverse transcription polymerase chain reaction (RT-PCR). Results: St-Ag enhanced cetuximab cytotoxic effect on all cells. LDH activity, as a result of cell death, was found the highest level at treatment of cetuximab with St-Ag in all cells. Both treatment increased caspase-3/7 activity which is apoptotic enzyme was found higher in A-549 cells than other cells. Also, treatment of cetuximab with St-Ag caused increasing Bax/Bcl-2 ratio in all cells. Cetuximab with St-Ag treatment increased glutathione peroxidase activity in all cells generating oxidative stress. Proliferating Cell Nuclear Antigen (PCNA), topoisomerase II-alpha (except R-H1299), cyclin D1 and D2 genes expression were decreased in all cells which explain the cell cycle inhibition effect. Conclusion: These findings suggest that treatment of cetuximab combined with St-Ag exhibit more carcinogen- esis reducing potential than cetuximab alone.