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    Öğe
    Mikotoksikoz tanısında kullanılabilecek lateral akım testi geliştirilmesi
    (2019) Dogan, Bora; Tamer, Uğur; Kalkanci, Ayse
    Bu projede mikotoksinlerden en sık görülen aflatoksin B1, okratoksin A ve fumonisin B1 varlıgını gösteren antijen-antikor temelli bir lateral akım çubugu gelistirilmesi amaçlanmıstır. Mikotoksikoz insanlarda ve hayvanlarda mikotoksin ile kontamine gıdaların tüketilmesi veya solunum yolundan alınması veya deriden direkt temas ile olusan hastalıgın adıdır. Insan ve hayvan serum örneklerinde mikotoksin varlıgının gösterilmesi için zor ve pahalı yöntemler kullanılmaktadır. Gıdalarda, özellikle süt ve sıvılarda mikotoksin varlıgının gösterilmesine yönelik hızlı kart testler ve ELISA testleri bulunmaktadır. Ayrıca gıdalarda kütle spektrometresi temelli gelismis laboratuvar testleri ile mikotoksinler gösterilebilir. Projemiz dahilinde gelistirdigimiz lateral akım çubugu, aynı anda üç mikotoksini serum örneklerinde gösterebilecek olması nedeniyle özgündür. Lateral akım çubugunun gelistirilmesi için mikotoksinlerden aflatoksin B1 monoklonal antikorları kırmızı renkli sellüloz nanopartiküllere, okratoksin A mavi renklilere, fumonisin B1 de yesil renklilere baglanmıstır. Antijen olarak mikotoksin standartları kullanılmıstır. Nitroselüloz test membranı üzerinde üç farklı renkte antijen-antikor bandı olusumunu aynı anda gösterebilecek sekilde bir lateral akım çubugu gelistirilmistir. Gelistirilen lateral akım çubugu önce mikotoksin standartları ile ardından mikotoksin eklenmis insan serumları ile test edilmistir. Aynı standartlar ve serum örnekleri ELISA yöntemiyle ve kütle spektrometresi (LCMS/ MS) ile ayrıca analiz edilmistir. Projenin hedefi olan üç farklı renkte bant olusturan çok hedefli (multipleks) bir mikotoksin tanı çubugu gelistirilmistir.
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    Öğe
    The effect of thymoquinone and propranolol combination on epidermoid laryngeal carcinoma cell
    (Springer, 2023) Sahin, Caner; Maytalman, Erkan; Samur, Dilara Nemutlu; Dogan, Bora
    Purpose We aimed to evaluate the effects of thymoquinone and propranolol on Hep-2 cells representing laryngeal Ca cell type in comparison with cisplatin. We also evaluated their combined effects. Methods Apoptotic effects were directly analyzed via mitochondrial membrane potential and caspase-3 assays. In addition, effects on apoptosis and cell cycle via Bcl-2, Bax, P53, and Cyclin D1 mRNA expressions and effects on angiogenesis via VEGFA mRNA expression were evaluated by RT-qPCR. Results According to our results, it was determined that the anticancer effects of thymoquinone on Hep-2 cells were higher than propranolol. Our JC-1 and caspase-3 results showed an effect close to cisplatin, especially for 50 mu M thymoquinone. Significant differences were also obtained in Bcl-2, Bax, P53, and cyclin D1 results for similar concentrations compared to the control. No effect of thymoquinone was seen for VEGFA. Propranolol alone had no significant effect on JC-1 and Caspase-3. Propranolol had an effect on Bcl-2, Bax mRNA expressions compared to the control, only at 250 mu M concentration. Propranolol and its combinations increased VEGFA mRNA expression-like cisplatin. Conclusion Thymoquinone induced apoptosis and blocked the cell cycle in Hep-2 cells. The effects of propranolol, which was reported to have an antiangiogenesis effect in some studies, on apoptosis and cell cycle were limited except at high concentrations. For this cell line, why propranolol causes an increase in VEGFA expression should be evaluated extensively. Thymoquinone shows promise for cancer therapy, but studies need to be designed in vivo to evaluate the effects more reliably.
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    Öğe
    West Nile virus seropositivity in Alanya, a coastal city in the Mediterranean region of Turkey
    (K Faisal Spec Hosp Res Centre, 2024) Bektore, Bayhan; Dogan, Bora; Ozkul, Akyut; Gozalan, Aysegul
    BACKGROUND: West Nile virus (WNV)-related illness is a global health problem. Understanding the seropositivity rates and identifying the risk factors related to WNV in various animal species including humans is crucial for the implementation of effective prevention strategies. OBJECTIVES: Assess the rate of seropositivity and the risk factors as-sociated with WNV seropositivity .DESIGN: Descriptive, cross-sectional SETTING: Microbiology and virology departments in a veterinary college PATIENTS AND METHODS: In a sample of healthy human participants in Alanya, located close to regions where WNV activity has been detected, anti-WNV IgG antibody detection was performed using enzyme-linked immunosorbent assays. The positive results were con-firmed by virus neutralization tests (VNTs). The sample was compared with a second group of age- and gender-matched healthy subjects selected from a previous cross-sectional study. MAIN OUTCOME MEASURES: Determination of the seropositivity and risk factors that were associated with WNV in healthy humans. SAMPLE SIZE: 87 in current study; 356 in previous study. RESULTS: The first group of 87, which had a high risk of encountering vector mosquitoes, had a positivity rate of 8% (7/87), whereas positivity in the second group was 4.5% (16/356; P=.181). In the entire sample, the anti-WNV IgG antibody was positive in 23 out of 443 (5.2%) sam-ples by the ELISA test. Among these 23 samples, ten were confirmed as positive using VNTs. Therefore, the WNV IgG seropositivity was 2.3% (10/442). Confirmed IgG seropositivity rates were higher among male (3.8%) than female participants (0.9%; P=.054) and among adults aged >= 45 years (4%) than those aged 18-44 years (0.8%; P=.048). CONCLUSION: This study highlights the presence of WNV infection in the research region. More comprehensive and multidisciplinary studies are required to increase our knowledge about this zoonotic infection including risk factors in line with the One Health approach LIMITATIONS: Small sample size.