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    Öğe
    Candida parapsilosis izolatlarının soğuk saklama koşullarına dayanıklılığı: On yıllık stoklama sonrası canlandırma
    (2020) Hoşbul, Tuğrul; Gümral, Ramazan; Bektöre, Bayhan; Tekin, Kemal; Şahiner, Fatih
    Amaç: Mikrobiyoloji laboratuvarlarında yürütülen araştırmalara farklı özelliklere sahip çok sayıdaki türün ve izolatın dâhil edilmesi çalışma verilerini değerli kılan önemli göstergelerden biridir. Bu amaçla her laboratuvar özgün bir suş koleksiyonu oluşturur. Bunun sürdürülebilirliği için tercih edilen saklama yöntemi izolatların uzun süreli saklanmasına ve hücrelerin canlı ve stabil kalmasına uygun olmalıdır. Bu çalışmada, Candida türlerinin soğukta saklama koşullarına olan dayanıklılıklarının tür düzeyinde incelenmesi amaçlanmıştır. Yöntem: Çalışmaya 10 yıldan daha uzun bir süredir laboratuvarımızda saklanan (-20°C’de boncuklu saklama tüpleri içerisinde) ve bu süre zarfında üzerlerinde herhangi bir canlandırma işlemi veya farklı bir çalışma yapılmayan, klinik örneklerden elde edilmiş 94 stok Candida izolatı dâhil edilmiştir. Çalışma örneklerinden sıvı ve katı besiyerlerine pasajlar alınarak canlanan suşların güncel rutin tanı yöntemleriyle doğrulaması yapılmıştır. Bulgular: Çalışma izolatlarında canlanma oranı Candida parapsilosis için %59.1 (13/22), Candida glabrata için %41.7 (5/12), Candida tropicalis için %25 (4/16) ve Candida albicans için %9.52 (4/42) olarak bulundu. C. parapsilosis izolatlarının canlı kalma oranı, C. albicans ve C. tropicalis izolatları ile karşılaştırıldığında istatistiksel olarak anlamlı derecede yüksek bulundu (sırasıyla p<0.0001 ve p=0.037). Sonuç: Bu çalışmada sunulan veriler yüksek sıcaklıklarda canlılığını sürdürebilen, soğuk koşullara adapte olan ve hastane ortamlarında uzun süre canlılığını sürdürebilen bir tür olan C. parapsilosis izolatlarının soğuk saklama koşullarına C. albicans başta olmak üzere C. tropicalis ve C. glabrata türlerinden daha dayanıklı olduğunu desteklemektedir.
  • Küçük Resim
    Öğe
    Evaluation of antibody response after COVID?19 vaccination of healthcare workers
    (2022) Uysal, Elif B.; Gümüş, Sibel; Bektöre, Bayhan; Bozkurt, Hale; Gözalan, Ayşegül
    The common goal of all vaccines developed against COVID-19, although they have been designed with different methods, is to develop an effective immunity and antibody response against SARS-CoV-2. However, the postvaccination immune response and antibody levels differ between individuals. This study examined the relationship between postvaccine seropositivity rates, age, gender, smoking, and body mass index (BMI), and antibody titers. A total of 314 healthcare workers (HCW) who were not previously infected with COVID-19 and who had received two doses of CoronaVac inactivated vaccine participated in the study. Seropositivity against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein was measured from the participants 4 weeks after the second dose of vaccine using the electrochemiluminescence (ECLIA) method. In addition, the antibody developed against the nucleocapsid protein (NCP) was evaluated and compared using Elecsys Anti-SARS-CoV-2 kit. One hundred and eighty-one of the participants were female (57.6%) with a median age of 39 (interquartile range [IQR], 10) and 133 (42.4%) were male with a median age of 41 (IQR, 11). 99.6% of the volunteers developed seropositivity 4 weeks after the second dose of vaccine. It was also observed that the rate of RBD protein antibody titer was >250 U/ml in smokers, which is quite low compared to nonsmokers (p = 0.032), and that high RBD antibody titers were proportionally lower in obese participants, according to BMI values, compared to those with normal BMI (49.5% and 9.9%). It was observed that seropositivity developed in almost all participants after the CoronaVac vaccine. However, it was determined that the antibody titer measured varied depending on factors such as smoking, BMI, and duration.
  • Küçük Resim
    Öğe
    Maldi-Tof MS Analysis of Long-Term Stored Stock Candida Strains Identified by Molecular and Conventional Methods
    (2021) Hoşbul, Tuğrul; Şahiner, Fatih; Gümral, Ramazan; Kaya, Sinem; Bektöre, Bayhan; Tekin, Kemal; Yıldıran, Şinasi Taner
    Objective: In recent years, the rapid and accurate identification of Candida isolates at species level has been of great importance for reasons such as increased incidence of infections due to Candida species and differing antifungal susceptibilities by species. Herein, we aimed to investigate the efficacy of the MALDI-TOF MS method in the identification of Candida clinical isolates stored at-20 degrees C for years. Material and Method: Twenty eight Candida strains stored at-20 degrees C for more than 10 years without any recultivation and identified by various methods such as API (R) ID 32C, CHROMagar Candida and PCR-RFLP were included in the study. Isolates were subcultured and analysed by MALDI-TOF MS. An unidentified isolate at the species level previously and another isolate with low MALDI-TOF MS score were analyzed by ITS sequencing. Additionally, MALDI-TOF MS scores of 57 Candida strains isolated from routine cultures were compared with study isolates. Results: Of them, 11 (39.3%) isolates were identified at the species level and 15 (53.6%) were identified at the genus level, while two isolates were outside the reliable identification limits by MALDI-TOF MS. MALDI-TOF MS yielded an accurate identification at the species level in all 28 isolates identified by various methods even though different scores were obtained. Conclusion: MALDI-TOF MS is a cost effective, easy to use, fast and reliable method compared with the conventional and molecular methods for identification of stock Candida isolates preserved for many years. In conclusion, although a limited number of stock Candida isolates were included in the study, low identification scores for Candida isolates may be valuable in the MALDI-TOF MS analysis.