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Öğe Agomelatine could prevent brain and cerebellum injury against LPS-induced neuroinflammation in rats(Academic Press Ltd- Elsevier Science Ltd, 2020) Savran, Meltem Karadeniz; Aslankoç, Rahime; Özmen, Özlem; Erzurumlu, Yalçın; Savas, Hasan Basri; Temel, Esra Nurlu; Bortepe, S.Sepsis, systemic hyper-inflammatory immune response, causes the increase of morbidity and mortality rates due to multi-organ diseases such as neurotoxicity. Lipopolysaccharide (LPS) induces inflammation, oxidative stress and apoptosis to cause brain damage. We aimed to evaluate the antioxidant, anti-inflammatory and antiapoptotic effects of Agomelatine (AGM) on LPS induced brain damage via NF-kB signaling. Twenty-four animals were divided into three groups as control, LPS (5 mg/kg) and LPS + AGM (20 mg/kg). Six hours after the all administrations, rats were sacrificed, brain tissues were collected for biochemical, histopathological and immunohistochemical analysis. In LPS group; total oxidant status (TOS), OSI index, Caspase-8 (Cas-8), NF-k beta levels increased and Total antioxidant status (TAS) levels decreased biochemically and Cas-8, haptoglobin and IL-10 expressions increased and sirtuin-1 (SIRT-1) levels decreased immunohistochemically. AGM treatment reversed these parameters except haptoglobin levels in hippocampus and SIRT-1 levels in cerebellum. Besides, AGM treatment blocked the phosphorylation of NF-kB biochemically and ameliorated increased the levels of hyperemia, edema and degenerative changes histopathologically. In conclusion, AGM enhanced SIRT-1 levels to negatively regulate the transcription and activation of p-NF-kB/p65 which caused to ameliorate inflammation, oxidative stress and apoptosis.Öğe Ameliorative effects of pregabalin on LPS induced endothelial and cardiac toxicity(Taylor & Francis Ltd, 2020) Aşçı, Halil; Özmen, Özlem; Erzurumlu, Yalçın; Savaş, Hasan Basri; Temel, Esra Nurlu; İçten, Pelin; Hasseyid, N.We investigated the antioxidant, anti-inflammatory and anti-apoptotic effects of pregabalin (PREG) on lipopolysaccharide (LPS) induced sepsis related cardiotoxicity via NF-k beta pathways. We used 24 female Wistar albino rats divided into three groups: control, LPS treated and LPS + PREG treated. Total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), tumor necrosis factor alpha (TNF-alpha), nuclear factor kappa beta (NF-k beta)/p65, p-NF-k beta/p65, caspase-3 (Cas-3) and cleaved Cas-3 were measured in cardiac tissues and creatine kinase MB (CKMB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) levels were measured in blood samples. Also, Cas-3, granulocyte-colony stimulating factors (G-CSF), interleukin-6 (IL-6), serum amyloid A (SAA) and inducible nitric oxide synthase (iNOS) were measured immunohistochemically in heart and aorta tissue. In the LPS group; the levels of CKMB, AST, LDH, TOS, OSI increased and TAS decreased. TNF-alpha, p-NF-k beta/p65 and Cas-3 protein levels also increased in the LPS group. Immunohistochemical evaluation of the heart and aorta revealed a significant increase in the levels of Cas-3, G-CSF, SAA, IL-6 and iNOS in the LPS group. PREG treatment restored all measurements to near normal. LPS induced cardiovascular toxicity was due to inflammation, oxidative stress and apoptosis. PREG ameliorated the damage by inhibition of NF-k beta phosphorylation.Öğe Effects of LDD and CAPE Administration on Total Antioxidant and Total Oxidant Levels in Experimental Periodontitis Model of Rat Brain(2022) Yiğit, Umut; Kırzıoğlu, Fatma Yeşim; Özmen, Özlem; Uğuz, AbdülhadiAim: Observing the effects of caffeic acid phenethyl ester (CAPE) and/or low dose doxycycline (LDD) on total antioxidant and oxidant status of brain in experimental periodontitis is the purpose of the study. Methods: 48 male Wistar albino rats were designed as the following: control group (C, n=8), periodontitis + CAPE group (PC, n=10), periodontitis + LDD (PD, n=10), periodontitis + LDD + CAPE group (PCD, n=10), and periodontitis group (P, n=10). The time period for the experiment was 14 days. 10 µmol/kg/day of CAPE was administered using an intraperitoneal injection (IP). 10 mg/kg/day of LDD was administered using an oral gavage method. Histopathological changes were evaluated. Results: Beneficial results were seen in all of the groups after LDD and/or CAPE administration on decreasing the alveolar bone loss level and oxidative stress. All of the experimental groups showed signs of periodontitis with alveolar bone loss. The P group leads with the most alveolar bone loss compared to the other periodontitis groups and the lowest group was the PC group in the periodontitis groups. The evolution of alveolar bone loss from high to low was that group P, group PD, group PCD, group PC, and group C (P < 0.05). However, there is no statistical difference between brain total antioxidant status and brain total oxidant status average values according to brain groups (p > 0.05). Conclusion: The combination of LDD and CAPE are not significantly different when applied alone or together on oxidative status. But both of the agents have beneficial effects on reducing the oxidative stress and tissue damages.Öğe Melatonin protects the heart and endothelium against high fructose corn syrup consumption-induced cardiovascular toxicity via SIRT-1 signaling(Sage Publications Ltd, 2019) Savran, Mehtap; Aşçı, Halil; Özmen, Özlem; Erzurumlu, Yalçın; Savaş, Hasan Basri; Sönmez, Yasin; Şahin, YaseminHigh fructose corn syrup (HFCS) has been shown to cause cardiovascular toxicity via oxidative stress and inflammation. The aim of this study is to demonstrate the protective effects of melatonin (MLT) against HFCS-induced endothelial and cardiac dysfunction via oxidative stress and inflammation. Thirty-two Sprague Dawley male rats were distributed into three groups as control, HFCS, and HFCS + MLT. HFCS form F55 was prepared as 20% fructose syrup solution and given to the rats through drinking water for 10 weeks, and MLT administrated 10 mg/kg/day orally for last 6 weeks in addition to F55. After decapitation, blood and half of the heart samples were collected for biochemical analysis and other half of the tissues for histopathological and immunohistochemical analysis. Aspartate transaminase, creatine kinase MB, lactate dehydrogenase, total oxidant status and oxidative stress index, and caspase-3 levels increased and total antioxidant status levels decreased significantly in HFCS group. MLT treatment reversed all these parameters. Histopathologically, hyperemia, endothelial cell damage and increased levels of angiogenin, C-reactive protein, inducible nitric oxide synthase, myeloperoxidase and decreased sirtuin-1 (SIRT-1) expressions were observed in HFCS group. MLT ameliorated all these changes. MLT has an anti-inflammatory, antioxidant, antiapoptotic effects on HFCS-induced cardiovascular toxicity through enhancing the expression of SIRT-1.Öğe Sıçanlarda indometazin ile indüklenmiş peptik ülser modelinde tarantula cubensis ekstraktının gastroprotektif etkisi(Alanya Alaaddin Keykubat Üniversitesi, 2020) Çankara, Fatma Nihan; Özmen, Özlem; Savaş, Hasan Basri; Şahin, Yasemin; Günaydın, CanerAmaç: Gastrik ülser, endüstrileşmiş dünyada çeşitli faktörler nedeniyle en sık görülen gastrointestinal hastalıktır. Bununla birlikte mide ülseri, gastrointestinal sağlığın korunması için genellikle tercih edilen proton pompa inhibitörleri (PPI) veya anti-asitler gibi birçok gastroprotektif farmakolojik ajanın kombine kullanılmasından sonra da nüks edebilmektedir. Bu kombinasyonlardaki beklenmedik olumsuz etkiler doğal ürünleri alternatif bir seçenek haline getirir. Bu nedenle, bu çalışmanın temel amacı, tek bir indometasin (40 mg/kg) ile oluşturulan deneysel peptik ülser modelinde doğal bileşiklerden biri olan Tarantula cubensis (TCE) ekstraktının etkisinin araştırılmasıdır.Yöntemler: İndometazin uygulanmasından önce iki doz TCE (0.2 ml/kg) s.k yoldan uygulanmıştır. Tek doz Indometazin (40 mg/kg) uygulamasından altı saat sonra anestezi altında mide dokusu biyokimyasal ve immünohistokimyasal analiz için çıkarılmıştır. Total antioksidan durum/total oksidan durum (TAS/TOS), prostaglandin E2 (PGE2) ve nükleer faktör kappa-B (NF-?B) düzeyleri doku homojenatlarında ELISA ile belirlenmiştir. Kaspaz-3, siklooksijenaz-2 (COX-2), indüklenebilir nitrik oksit sentaz (iNOS) ve tümör nekroz faktörü-alfa (TNF-?) immünohistokimya ile görüntülenmiştir.Bulgular: TCE ile ön tedavi PGE2 düzeylerini artırdı ve toplam oksidatif durumu (TAS / TOS) azaltmıştır. Ek olarak, TCE indometazin uygulamasına bağlı olarak NF-?B düzeylerindeki artışı hafifletmiştir. Histopatolojik ve immünboyama sonuçları, TCE'nin, indometazin uygulamasının sonuçları olan kaspaz-3, COX-2, iNOS ve TNF-?'nın yüksek immünoreaktivitesini azalttığını göstermiştir.Sonuç: Çalışmamız TCE tedavisinin, antioksidan ve antiinflamatuvar etkiler yoluyla indometazine bağlı peptik ülserleri iyileştirdiğini göstermiştir.Öğe The differentiation of metastatic mediastinal lymph nodes From benign hypermetabolic lesions(2022) Bulut, Sertan; Çelik, Deniz; Karamanlı, Harun; Aktaş, Zafer; Özmen, Özlem; Ertürk, Hakan; Gürçay, Nesrin; Biber, ÇiğdemBackground Anthracosis may cause a positron emission tomography/computed tomography (PET/CT) false positivity in mediastinal and hilar lymph nodes. We aimed to evaluate the radiological features and the maximum standardized uptake values (SUVmax) of the mediastinal lymph nodes with anthracosis or squamous cell lung cancer metastasized. Methodology Patients diagnosed with anthracosis or squamous cell lung cancer with endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) between January 1, 2015, and November 15, 2020, in a tertiary hospital were enrolled. The squamous cell subtype of lung cancer was selected due to its association with tobacco use, biomass, and air pollution. Anthracosis may occur due to the same etiologic reasons. Results A total of 190 patients met the study enrollment criteria, of which 86 were diagnosed with anthracosis and 33 with squamous cell lung cancer lymph metastasis. Median values for short axis, long axis, SUVmax, shape features, and presence of calcification were found significantly different between the groups. In receiver operating characteristic (ROC) analysis, the SUVmax cut-off value was calculated as 6.61. With this cutoff value, the negative predictive value (NPV) was 92.5% and the positive predictive value (PPV) was 54% for differentiating anthracosis and malignant lymph nodes metastasis. Conclusions We conclude that the evaluation of the shape and metabolic activities of the anthracotic lymph nodes detected by PET/CT together with EBUS-TBNA granted a more accurate staging of the patients and more cancer patients will benefit from surgical treatment.Öğe The impact of prophylactic lacosamide on LPS-induced neuroinflammation in aged rats(Springer/Plenum Publishers, 2019) Savran, Mehtap; Özmen, Özlem; Erzurumlu, Yalçın; Savaş, Hasri Basri; Aşçı, S.; Kaynak, M.Sepsis-induced central nervous system damage is called sepsis-associated encephalopathy (SAE). In addition to neuroinflammation, oxidative stress and apoptosis act in the development of SAE. In the current study, we evaluated the protective effects of lacosamide (LCM) on neuroinflammation induced by lipopolysaccharide (LPS). Twenty-four Wistar albino rats were divided into 3 groups as controls, LPS group (5 mg/kg i.p.), and LPS plus LCM group (5 mg/kg i.p and 40 mg/kg i.p, respectively). In the rat brain, LPS-induced tissue damage was revealed histopathologically as hyperemia and microhemorrhages. LCM pretreatment ameliorated these histopathological changes. LPS decreased brain TAS levels and significantly increased MDA, CRP, HSP, IL-1 beta, and TNF-alpha expressions in the cortex, hippocampus, and cerebellum. Western analysis revealed increased brain tissue levels of TNF-alpha, NF-K beta, and caspase-3 following LPS. Prophylactic LCM treatment reversed these parameters including oxidative stress, inflammation, and apoptosis in the cortex, hippocampus, and cerebellum.
