Maytalman, ErkanSamur, Dilara Nemutlu2026-01-242026-01-2420241355-008X1559-0100https://doi.org/10.1007/s12020-024-03822-3https://hdl.handle.net/20.500.12868/5575PurposeSome evidence that non-steroidal anti-inflammatory drugs have neuroprotective effects indicates their potential for use in a new field. However, their effects on hormone secretion have yet to be adequately discovered. Therefore, we aimed to evaluate the effects of metamizole and indomethacin on neuronal markers as well as the GnRH expression in the GT1-7 cell line.MethodsThe effects of these drugs on proliferation were evaluated by MTT analysis. The effect of 10-50-250 mu M concentrations of the drugs also on the expression of neuronal factors and markers, including NGF, nestin and beta III Tubulin, and additionally GnRH, was determined by the RT-qPCR method.ResultsNGF and nestin mRNA expressions were increased in all concentrations of both metamizole and indomethacin. No changes were detected in beta III Tubulin. While metamizole showed an increase in GnRH mRNA expression, there was no change at 10 and 50 mu M concentrations of indomethacin, but a remarkable decrease was observed at 250 mu M concentrations.ConclusionsThe results of our study showing an increase in the expression of neuronal factors reveal that metamizole and indomethacin may have possible neuroprotective effects. Moreover, the effects on the GnRH expression appear to be different. Animal models are required to confirm these effects of NSAIDs on neurons.eninfo:eu-repo/semantics/closedAccessMetamizoleIndomethacinGT1-7 cell lineNeuronal markersGnRHArticle10.1007/s12020-024-03822-385313271336386255032-s2.0-85190532301Q2WOS:001289493800031Q2