Deniz, AlparslanKarasu, TuncaOzgur, ErdoganUzun, Lokman2026-01-242026-01-2420251567-53941878-562Xhttps://doi.org/10.1016/j.bioelechem.2024.108820https://hdl.handle.net/20.500.12868/5648Herein, a conjugated conducting polymer-based impedimetric aptasensor has been developed to detect beta-human chorionic gonadotropin (bHCG), the one of the important biomarkers in gynecology, from synthetic human urine samples. In this context, gold electrodes were, firstly coated with pyrrole and pyrrole-3-carboxylic acid to obtain the poly(pyrrole-pyrrole-3-carboxylic acid) [poly(Py-PyCOOH)] conductive copolymer by cyclic voltammetry (CV). Then, bHCG-specific peptide aptamer was covalently linked onto the surface via applying a well-known carbodiimide-succinimide chemistry. The sensor developed was characterized to confirm modification steps via both electrochemical methods including CV, electrochemical impedance spectroscopy (EIS), and chronoamperometry and physico-chemically via attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), atomic force microscope (AFM), and contact angle measurements (CA). The analytical performance of the sensor was evaluated in the concentration range from 1 mu g/mL to 100 mu g/mL for successful detection of bHCG even in the presence of interference agents. The results have also revealed that the sensor could be classified as a promising alternative to its benchmark commercial clinical methods due its superior properties such as cost-friendliness, easy-to-prepare, stable, robust, and selectivity / sensitivity.eninfo:eu-repo/semantics/closedAccessPolypyrroleAptasensorHuman chorionic gonadotropinImpedimetric detectionUrineArticle10.1016/j.bioelechem.2024.108820161392991862-s2.0-85204093537Q1WOS:001320215000001Q1