Potential protective effect of astaxanthin on ovary ischemia-reperfusion injury
Özet
Objective(s): We thought that astaxanthin (ASX) might be a protective agent in oxidative stress
damage that develops against ischemia and reperfusion injury in the rat ovary.
Materials and Methods: The experimental groups consisted of healthy, I (Ischemia), I+ASX50, I+ASX100,
I/R (Ischemia/Reperfusion), I/R+ ASX50, and I/R+ ASX100. Vascular clamps were applied to the ovaries
for 3 hr to induce ischemia. For the reperfusion groups, the clamps were opened and blood flow was
restored to the ovaries for 3 hr. At the end of the experiment, biochemical, histopathological, and
immunohistochemical analyses were made from the tissue samples taken.
Results: While MDA levels increased significantly in I and I/R groups, SOD levels decreased. It was
found that ASX significantly decreased MDA levels and increased SOD activity in treatment groups
depending on the dose. Caspase 3, IL-1 β, and IL-6 expressions were severely increased in ischemia
and I/R groups, while the severity of I+ASX50 and I/R+ASX100 immunoreactivity was decreased.
While severe hemorrhage areas were observed in I and IR groups, minimal hemorrhage areas were
observed in the treatment groups, especially in I/R+ASX100 groups. In addition, inflammatory cells
and necrotic cells in the I/R group were not observed in I/R+ASX50 and I/R+ASX100 groups.
Conclusion: As a result, it was determined that ASX has a strong protective role against oxidative
damage in the treatment of ovarian ischemia-reperfusion injury
