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dc.contributor.authorGiray, Aslı
dc.date.accessioned2024-10-24T06:38:31Z
dc.date.available2024-10-24T06:38:31Z
dc.date.issued2022en_US
dc.identifier.urihttps://pubmed.ncbi.nlm.nih.gov/34865603/
dc.identifier.urihttps://hdl.handle.net/20.500.12868/2488
dc.description.abstractVitamin A prevents eye problems, blindness and skin problems by strengthening the immune system. Vitamin E is a nutrient that has important roles in many areas such as skin health, eye health and hormonal order. Vitreoscilla hemoglobin (VHb) gives an advantage in later phases of grown conditions to cells. In this study, the intracellular and extracellular production of vitamin A and E in E. herbicola and its recombinant strains (vgb- and vgb+) in the three different M9 mediums with supplemented 0.1% glucose, 0.1% fructose and 0.1% sucrose was investigated. Additionally, the viable cell number and total cell mass (OD600) were measured by the host and the recombinant bacteria in these mediums. The VHb gene expression in E. herbicola enhanced vitamin A under different carbon conditionals. Especially, in the vgb + strain (carrying vgb gene) the production of total vitamin in 0.1% glucose medium was recorded as 0.14 µg/ml, while the production in fructose and sucrose media was recorded as 0.07 µg/ml. The production of intracellular vitamin E in the host strain (0.025 µg/ml) was about 13-fold (0.002 µg/ml) higher than vgb + recombinant strain in 0.1% fructose. The vgb + strain showed about 2-fold higher extracellular vitamin E production than the host strain.en_US
dc.language.isoengen_US
dc.relation.isversionof10.1080/10826068.2021.2004548.en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiotechnologyen_US
dc.subjectE. herbicolaen_US
dc.subjectVHb hemoglobinen_US
dc.subjectVitamin Aen_US
dc.subjectVitamin Een_US
dc.titleProduction of vitamin A and vitamin E: expression of vitreoscilla hemoglobin gene in Erwinia herbicolaen_US
dc.typearticleen_US
dc.contributor.departmentALKÜ, Fakülteler, Rafet Kayış Mühendislik Fakültesi, Genetik ve Biyomühendislik Bölümüen_US
dc.identifier.volume52en_US
dc.identifier.issue8en_US
dc.identifier.startpage894en_US
dc.identifier.endpage902en_US
dc.relation.journalPrep Biochem Biotechnolen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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