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dc.contributor.authorGiray, Aslı
dc.contributor.authorPekdemir, Sibel
dc.date.accessioned2022-11-30T05:45:28Z
dc.date.available2022-11-30T05:45:28Z
dc.date.issued2022en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1127852/biotechnological-micronutrient-production-recombinant-dna-technology-based-vitamin-a-synthesis
dc.identifier.urihttps://hdl.handle.net/20.500.12868/2060
dc.description.abstractVitamin A is an essential micronutrient and has important functions such as vision, growth, reproduction embryogenesis, cellular differentiation, and proliferation, immune function and epithelial protector in the organism. Biotechnological production of vitamins is increasing due to their advantages and significant advances. The vitreoscilla hemoglobin (VHb) gene is extremely effective in binding oxygen and conducting it under hypoxic conditions. In this study, the production of vitamin A in E. herbicola (wild type) and its recombinant strains was investigated in LB medium and M9 medium (containing high concentrations (1%) of different carbon sources). The maximum production of vitamin A of the recombinant strain with the hemoglobin gene (vgb+) was observed in including glucose and sucrose M9 medium and their total product levels in vgb+ recombinant strain were 0.14 μg/ml and 0.1 μg/ml, respectively. The vitamin A production in the M9 medium with glucose and sucrose were 2-fold and 1.4- fold higher than that of the wild strain, respectively. The extracellular product level (0.07 μg/ml) in LB was 7-fold higher than wild strain at 48 h. These results reveal that the expression of VHb in E. herbicola in the both LB and M9 medium (containing 1% glucose and 1% sucrose, specially) increase the vitamin A production.en_US
dc.language.isoturen_US
dc.relation.isversionof10.24925/turjaf.v10i8.1420-1425.5069en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBiotechnological Productionen_US
dc.subjectVitamin Aen_US
dc.subjectVitreoscilla Hemoglobin (VHb)en_US
dc.subjectErwinia Herbicolaen_US
dc.subjectMicronutrienten_US
dc.titleBiotechnological Micronutrient Production: Recombinant DNA Technology- Based Vitamin A Synthesisen_US
dc.typearticleen_US
dc.contributor.departmentALKÜ, Fakülteler, Rafet Kayış Mühendislik Fakültesi, Genetik ve Biyomühendislik Bölümüen_US
dc.identifier.volume10en_US
dc.identifier.issue8en_US
dc.identifier.startpage1420en_US
dc.identifier.endpage1425en_US
dc.relation.journalTürk Tarım - Gıda Bilim ve Teknoloji Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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