Effects of LDD and CAPE Administration on Total Antioxidant and Total Oxidant Levels in Experimental Periodontitis Model of Rat Brain

Abstract

Aim: Observing the effects of caffeic acid phenethyl ester (CAPE) and/or low dose doxycycline (LDD) on total antioxidant and oxidant status of brain in experimental periodontitis is the purpose of the study. Methods: 48 male Wistar albino rats were designed as the following: control group (C, n=8), periodontitis + CAPE group (PC, n=10), periodontitis + LDD (PD, n=10), periodontitis + LDD + CAPE group (PCD, n=10), and periodontitis group (P, n=10). The time period for the experiment was 14 days. 10 µmol/kg/day of CAPE was administered using an intraperitoneal injection (IP). 10 mg/kg/day of LDD was administered using an oral gavage method. Histopathological changes were evaluated. Results: Beneficial results were seen in all of the groups after LDD and/or CAPE administration on decreasing the alveolar bone loss level and oxidative stress. All of the experimental groups showed signs of periodontitis with alveolar bone loss. The P group leads with the most alveolar bone loss compared to the other periodontitis groups and the lowest group was the PC group in the periodontitis groups. The evolution of alveolar bone loss from high to low was that group P, group PD, group PCD, group PC, and group C (P < 0.05). However, there is no statistical difference between brain total antioxidant status and brain total oxidant status average values according to brain groups (p > 0.05). Conclusion: The combination of LDD and CAPE are not significantly different when applied alone or together on oxidative status. But both of the agents have beneficial effects on reducing the oxidative stress and tissue damages.