The Protective Effects Of Beta Glucan Against Experimental Renal Ischemia Reperfusion Injury
Abstract
Aim: This study aimed to investigate the possible protective effects of beta-glucan
against oxidative stress caused by ischemia and reperfusion injury in kidney tissue.
Materials and methods: In the study, 30 male Wistar albino rats weighing 300-350g
were used (n=10). Rats were randomly grouped into three groups of Sham control,
ischemia reperfusion group (IR), ischemia reperfusion + beta glucan group (IR +
BG). Sham group had left nephrectomy, the right kidney taken for histopathologic and
biochemical examination. After left nephrectomy in IR group, ischemia procedure was
applied for 45 minutes via nontraumatic microvascular clamp, then reperfusion was
applied for 60 minutes in the right kidney. In the IR+BG group, rats were administered
100 mg/kg beta glucan via gastric gavage for 10 days. Reperfusion was applied for
60 minutes after 45 minutes of ischemia to the right kidney under anesthesia.
Results: As a result of biochemical examination MDA values showed a significant
increase in IR group compared to Sham group (p<0,05). In IR+BG group, there was a
significant decrease compared to IR group (p<0,05). Tissue MPO values in IR group
showed a significant increase compared to Sham group, whereas in the IR+BG group
there was not a significant decrease. Also, there was not a significant difference in
tissue catalase levels. Tissue GSH values showed a significant decrease in IR group
compared to Sham group (p<0,05). In the IR+BG group a significant increase was
found compared to IR group (p<0,05). Less damage has been revealed in the IR+BG
group compared to IR group in histopathologic examination.
Conclusion: All these data show that beta glucan may have an antioxidant effect on
renal ischemia reperfusion injury
Source
Acta Medica AlanyaVolume
6Issue
1URI
https://dergipark.org.tr/tr/download/article-file/1980059https://hdl.handle.net/20.500.12868/1753