Molecular characterization of carbapenem-resistant acinetobacter baumannii blood culture isolates from three hospitals in Turkey
dc.contributor.author | Gözalan, Ayşegül | |
dc.contributor.author | Ünaldı, Özlem | |
dc.contributor.author | Güldemir, Dilek | |
dc.contributor.author | Aydoğan, Sibel | |
dc.contributor.author | Kuzucu, Çiğdem | |
dc.contributor.author | Çakırlar, Fatma Köksal | |
dc.contributor.author | Açıkgöz, Ziya Cibali | |
dc.contributor.author | Durmaz, Rıza | |
dc.date.accessioned | 2022-09-22T06:12:27Z | |
dc.date.available | 2022-09-22T06:12:27Z | |
dc.date.issued | 2021 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.12868/1645 | |
dc.description.abstract | We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals in Turkey in 2016. Molecular epidemiological characteristics of the isolates were examined using pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, and rpoB). Multiplex PCR was used to investigate the carbapenemase genes, including bla(OXA-23-like),bla(OXA-24-like), bla(OXA-48-like), bla(OXA-58-like), bla(IMP), bla(VIM) and bla(NDm). PFGE genotyping yielded 92 pulsotypes with a clustering ratio of 69.7%. As per a >= 85% similarity coefficient, 159 (90.9%) isolates were found to be clonally related. The bla(OXA-)(23-like) and bla(OXA-)(58)-like genes were identified in 100% and 28.2% of the isolates, respectively. The bla(NDM) gene was identified in two isolates. The MLST analysis included 54 isolates with different pulsotypes, and 29 sequence types (STs). Most of the isolates (n = 36) belonged to the clonal complex (CC)2, one isolate belonged to CC1, and one isolate belonged to CC164. Sixteen new STs (ST1235-ST1250) were identified. Identifying both global ST2 and a large number of new STs, revealed high genetic diversity in A. baumannii isolates in the study population. | en_US |
dc.language.iso | eng | en_US |
dc.relation.isversionof | 10.7883/yoken.JJID.2020.478 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Escherichia-coli | en_US |
dc.subject | Beta-Lacramases | en_US |
dc.subject | Antimicrobial Resistance | en_US |
dc.subject | Mediterranean Countries | en_US |
dc.subject | High Prevalence | en_US |
dc.subject | Multiplex PCR | en_US |
dc.subject | Identification | en_US |
dc.subject | Genes | en_US |
dc.subject | Infections | en_US |
dc.subject | Diversity | en_US |
dc.title | Molecular characterization of carbapenem-resistant acinetobacter baumannii blood culture isolates from three hospitals in Turkey | en_US |
dc.type | article | en_US |
dc.contributor.department | ALKÜ, Fakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü | en_US |
dc.identifier.volume | 74 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.startpage | 200 | en_US |
dc.identifier.endpage | 208 | en_US |
dc.relation.journal | Japanese Journal of Infectious Diseases | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
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