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dc.contributor.authorYegani, Arash Alizadeh
dc.contributor.authorMaytalman, Erkan
dc.contributor.authorKozanoğlu, İlknur
dc.contributor.authorTerzi, Menderes Yusuf
dc.contributor.authorAksu, Fazilet
dc.date.accessioned2021-02-19T21:16:28Z
dc.date.available2021-02-19T21:16:28Z
dc.date.issued2020
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.urihttps://doi.org/10.22038/IJBMS.2019.38582.9151
dc.identifier.urihttps://hdl.handle.net/20.500.12868/441
dc.descriptionWOS: 000498871600017en_US
dc.descriptionPubMed: 32405354en_US
dc.description.abstractObjective(s): Cells perform their functional activities by communicating with each other through endogenous substances and receptors. Post-translation, stem cells function properly in new host tissue by carrying specific cell surface receptors. We aimed to characterize muscarinic receptor subtypes in mesenchymal stem cells (MSCs) together with osteogenic and adipogenic differentiation markers. Materials and Methods: mRNA levels of 5 muscarinic receptor subtypes (CHRM1 to 5), BMP-6, and PPAR gamma during osteogenic and adipogenic differentiation, under the effect of atropine blockade, were measured in MSCs obtained from human fetal membrane (FM) and bone marrow (BM). Additionally, the effect of atropine on differentiation in the 1st, 2nd, and 3rd passages of MSCs, obtained from human FM and BM, were analyzed by RT-qPCR. Results: CHRM1 mRNA levels increased in the FM group, while decreasing in the BM group. We found significant decreases in CHRM3 and CHRM5 mRNA levels in FM and BM groups, respectively. Atropine had variable effects based on cell source and receptor type. BMP-6 mRNA levels in differentiated osteogenic cells increased significantly compared to undifferentiated cells in both FM and BM groups. In MSCs derived from both sources, PPAR gamma mRNA levels in differentiated adipogenic cells increased significantly. Atropine showed no effect on MSCs differentiation. Conclusion: These results indicate that expressions of muscarinic receptors in MSCs derived from BM and FM can vary and these cells keep the potential of osteogenic and adipogenic differentiation in vitro. Besides, atropine had no effect on adipogenic and osteogenic differentiation of MSCs.en_US
dc.description.sponsorshipCukurova University, Scientific Research Projects Fund, Adana, Turkey [TF2014D2]en_US
dc.description.sponsorshipThe results presented in this paper were part of a student thesis. The authors express their great gratitude to Canan Cansun and Nilay Oktar for their invaluable help in the RT-qPCR technique. This study was supported by Cukurova University, Scientific Research Projects Fund, Adana, Turkey (project no: TF2014D2).en_US
dc.language.isoengen_US
dc.publisherMashhad Univ Med Sciencesen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAtropineen_US
dc.subjectBone marrowen_US
dc.subjectCell differentiationen_US
dc.subjectGene expressionen_US
dc.subjectHuman placentaen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectMuscarinic receptorsen_US
dc.titleBiological behaviors of muscarinic receptors in mesenchymal stem cells derived from human placenta and bone marrowen_US
dc.typearticleen_US
dc.contributor.departmentALKÜen_US
dc.contributor.institutionauthor0-belirlenecek
dc.identifier.doi10.22038/IJBMS.2019.38582.9151
dc.identifier.volume23en_US
dc.identifier.issue1en_US
dc.identifier.startpage124en_US
dc.identifier.endpage132en_US
dc.relation.journalIranian Journal of Basic Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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