Cilostazol induces angiogenesis and regulates oxidative stress in adose-dependent manner: A chorioallantoic membrane study

Abstract

Background: In this study, we aimed to investigate the effects ofcilostazol on angiogenesis and oxidative stress using the chorioallantoicmembrane model. Methods: In this experimental study, the Ross 308 chick embryos wereused. The negative control group (n=10) received no intervention. Thepositive control group (n=10) consisted of eggs treated with epidermalgrowth factor for inducing angiogenesis. Three cilostazol groups weredesigned with 10-7 (n=10), 10-6 (n=10), and 10-5 (n=10) M concentrations.Each egg was punctured on the sixth day of incubation, and drugpellets were introduced to the positive control and drug groups at theprespecified doses. Vascular development was evaluated on the eighthday of application. The total oxidant status, total antioxidant capacity,and oxidative stress index levels were determined from albumen liquidsobtained with a syringe before and after drug application. Results: Lower oxidative stress index levels were obtained fromthe positive control and cilostazol groups compared to the negativecontrol albumens (p=0.001). The increments in vascular junctions andnewly developed vascular nodules were evaluated in drug-free anddrug-applied chorioallantoic membranes. The highest activity wasobtained in the 10-7 M concentration cilostazol group. An increasedangiogenic activity was detected in all drug groups in each concentrationcompared to the negative control group (p=0.001). Angiogenic activitywas similar in all the cilostazol-treated groups (p=0.43).Conclusion: Cilostazol has a positive stimulant effect on angiogenesisand it seems to suppress oxidative stress during embryonic growth.Cilostazol exerts these effects significantly and similarly at differentdoses.